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Host-specific differences in the contribution of an extended spectrum β-lactamase (ESBL) IncI1 plasmid to intestinal colonisation by Escherichia coli O104:H4

Giles, M, Cawthraw, S, AbuOun, M, Thomas, C, Munera, D, Waldor, M, La Ragione, Roberto and Ritchie, Jennifer (2018) Host-specific differences in the contribution of an extended spectrum β-lactamase (ESBL) IncI1 plasmid to intestinal colonisation by Escherichia coli O104:H4 Journal of Antimicrobial Chemotherapy, 73 (6). pp. 1579-1585.

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Abstract

Objectives.

To assess stability and contribution of a large extended spectrum β-lactamase (ESBL)-containing IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4 in two different mammalian hosts.

Methods.

Specific-pathogen-free 3-day old New Zealand White rabbits and conventionally-reared 6-week-old weaned lambs were orally infected with wild-type E. coli O104:H4 or the ESBL-plasmid cured derivative, and the recovery of bacteria in intestinal homogenates and faeces monitored over time.

Results.

Carriage of the ESBL plasmid had differing impacts on E. coli O104:H4 colonisation of the two experimental hosts. The plasmid cured strain was recovered at significantly higher levels than wild type during late-stage colonization of rabbits, but at lower levels than wildtype in sheep. Regardless of the animal host, the ESBL plasmid was stably maintained in virtually all in vivo passaged bacteria that were examined.

Conclusions.

These findings suggest that carriage of ESBL plasmids has distinct effects on the host bacterium depending upon the animal species it encounters and demonstrates that, as for E. coli O157:H7, ruminants could represent a potential transmission reservoir.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Giles, M
Cawthraw, S
AbuOun, M
Thomas, C
Munera, D
Waldor, M
La Ragione, RobertoR.Laragione@surrey.ac.uk
Ritchie, Jenniferj.ritchie@surrey.ac.uk
Date : 28 February 2018
Identification Number : 10.1093/jac/dky037
Copyright Disclaimer : This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Antimicrobial Chemotherapy, following peer review. The version of record: M Giles, S A Cawthraw, M AbuOun, C M Thomas, D Munera, M K Waldor, R M La Ragione, J M Ritchie; Host-specific differences in the contribution of an ESBL IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4, Journal of Antimicrobial Chemotherapy, Volume 73, Issue 6, 1 June 2018, Pages 1579–1585, https://doi.org/10.1093/jac/dky037 is available online at:https://academic.oup.com/jac/article-abstract/73/6/1579/4913708?redirectedFrom=fulltext
Depositing User : Melanie Hughes
Date Deposited : 30 Jan 2018 08:45
Last Modified : 22 May 2018 17:44
URI: http://epubs.surrey.ac.uk/id/eprint/845710

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