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The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction

Ferguson, Richard A., Hunt, Julie, Lewis, Mark P., Martin, Neil R. W., Player, Darren J., Stangier, Carolin, Taylor, Conor W. and Turner, Mark C. (2018) The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction European Journal of Sport Science, 18 (3). pp. 397-406.

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Abstract

This study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT–PCR. Protein phosphorylation/expression was determined using Western blot. p38MAPK phosphorylation was greater (p = 0.05) at 2 h following BFR (1.3 ± 0.8) compared to CON (0.4 ± 0.3). AMPK phosphorylation remained unchanged. PGC-1α mRNA expression increased at 2 h (5.9 ± 1.3 vs. 2.1 ± 0.8; p = 0.03) and 4 h (3.2 ± 0.8 vs. 1.5 ± 0.4; p = 0.03) following BFR exercise with no change in CON. PGC-1α protein expression did not change following either exercise. BFR exercise enhanced mRNA expression of vascular endothelial growth factor (VEGF) at 2 h (5.2 ± 2.8 vs 1.7 ± 1.1; p = .02) and 4 h (6.8 ± 4.9 vs. 2.5 ± 2.7; p = .01) compared to CON. mRNA expression of VEGF-R2 and hypoxia-inducible factor 1α increased following BFR exercise but only eNOS were enhanced relative to CON. Matrix metalloproteinase-9 mRNA expression was not altered in response to either exercise. Acute low-load resistance exercise with BFR provides a targeted angiogenic response potentially mediated through enhanced ischaemic and shear stress stimuli.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Ferguson, Richard A.
Hunt, Juliej.hunt@surrey.ac.uk
Lewis, Mark P.
Martin, Neil R. W.
Player, Darren J.
Stangier, Carolin
Taylor, Conor W.
Turner, Mark C.
Date : 17 January 2018
Identification Number : 10.1080/17461391.2017.1422281
Copyright Disclaimer : © 2018 European College of Sport Science
Uncontrolled Keywords : BFR; Kaatsu; Resistance training; Capillaries; Hypoxia
Depositing User : Clive Harris
Date Deposited : 24 Jan 2018 15:53
Last Modified : 09 May 2018 12:31
URI: http://epubs.surrey.ac.uk/id/eprint/845689

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