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An aged canid with behavioural deficits exhibits blood and cerebrospinal fluid amyloid beta oligomers

Rusbridge, Clare, Salguero Bodes, Francisco, David, Monique A., Faller, Kiterie, Bras, Jose T., Guerreiro, Rita, Richard-Loendt, Angela C., Grainger, Duncan, Head, Elizabeth, Brandner, Sebastian G. , Summers, Brian, Hardy, John and Tayebi, Mourad (2018) An aged canid with behavioural deficits exhibits blood and cerebrospinal fluid amyloid beta oligomers Frontiers in Aging Neuroscience, 10, 7. pp. 1-8.

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Abstract

Many of the molecular and pathological features associated with human Alzheimer disease (AD) are mirrored in the naturally occurring age-associated neuropathology in the canine species. In aged dogs with declining learned behaviour and memory the severity of cognitive dysfunction parallels the progressive build up and location of Aβ in the brain. The main aim of this work was to study the biological behaviour of soluble oligomers isolated from an aged dog with cognitive dysfunction through investigating their interaction with a human cell line and synthetic Aβ peptides. We report that soluble oligomers were specifically detected in the dog’s blood and cerebrospinal fluid via anti-oligomer- and anti-Aβ specific binders. Importantly, our results reveal the potent neurotoxic effects of the dog’s cerebrospinal fluid on cell viability and the seeding efficiency of the cerebrospinal fluid-borne soluble oligomers on the thermodynamic activity and the aggregation kinetics of synthetic human Aβ. The value of further characterising the naturally occurring Alzheimer-like neuropathology in dogs using genetic and molecular tools is discussed.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Rusbridge, Clarec.rusbridge@surrey.ac.uk
Salguero Bodes, Franciscof.salguerobodes@surrey.ac.uk
David, Monique A.
Faller, Kiterie
Bras, Jose T.
Guerreiro, Rita
Richard-Loendt, Angela C.
Grainger, Duncan
Head, Elizabeth
Brandner, Sebastian G.
Summers, Brian
Hardy, John
Tayebi, Mourad
Date : 30 January 2018
Identification Number : 10.3389/fnagi.2018.00007
Copyright Disclaimer : Copyright © 2018 Rusbridge, Salguero, David, Faller, Bras, Guerreiro, Richard-Londt, Grainger, Head, Brandner, Summers, Hardy and Tayebi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Contributors :
ContributionNameEmailORCID
http://www.loc.gov/loc.terms/relators/EDTInestrosa, Nibaldo C.
Uncontrolled Keywords : Alzheimer Disease; Canine; Amyloid beta oligomers; Neuropathology; Protein aggregation; Neurotoxicity
Additional Information : The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnagi.2018.00007/full#supplementary-material
Depositing User : Clive Harris
Date Deposited : 16 Jan 2018 11:25
Last Modified : 08 May 2018 08:50
URI: http://epubs.surrey.ac.uk/id/eprint/845617

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