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Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle

Villarreal-Ramos, Bernardo, Berg, Stefan, Whelan, Adam, Holbert, Sebastien, Carreras, Florence, Salguero Bodes, Francisco, Khatri, Bhagwati, Malone, Kerri M., Rue-Albrecht, Kevin, Shaughnessy, Ronan , Smyth, Alicia M., Ameni, Gobena, Aseffa, Abraham, Sarradin, Pierre, Winter, Nathalie, Vordermeier, Martin and Gordon, Stephen V. (2018) Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle Scientific Reports, 8, 894.

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Abstract

The Mycobacterium tuberculosis complex (MTBC) is the collective term given to the group of bacteria that cause tuberculosis (TB) in mammals. It has been reported that M. tuberculosis H37Rv, a standard reference MTBC strain, is attenuated in cattle compared to Mycobacterium bovis. However, as M. tuberculosis H37Rv was isolated in the early 1930s, and genetic variants are known to exist, we sought to revisit this question of attenuation of M. tuberculosis for cattle by performing a bovine experimental infection with a recent M. tuberculosis isolate. Here we report infection of cattle using M. bovis AF2122/97, M. tuberculosis H37Rv, and M. tuberculosis BTB1558, the latter isolated in 2008 during a TB surveillance project in Ethiopian cattle. We show that both M. tuberculosis strains caused reduced gross and histopathology in cattle compared to M. bovis. Using M. tuberculosis H37Rv and M. bovis AF2122/97 as the extremes in terms of infection outcome, we used RNA-Seq analysis to explore differences in the peripheral response to infection as a route to identify biomarkers of progressive disease in contrast to a more quiescent, latent infection. Our work shows the attenuation of M. tuberculosis strains for cattle, and emphasizes the potential of the bovine model as a ‘One Health’ approach to inform human TB biomarker development and post-exposure vaccine development.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Villarreal-Ramos, Bernardo
Berg, Stefan
Whelan, Adam
Holbert, Sebastien
Carreras, Florence
Salguero Bodes, Franciscof.salguerobodes@surrey.ac.uk
Khatri, Bhagwati
Malone, Kerri M.
Rue-Albrecht, Kevin
Shaughnessy, Ronan
Smyth, Alicia M.
Ameni, Gobena
Aseffa, Abraham
Sarradin, Pierre
Winter, Nathalie
Vordermeier, Martin
Gordon, Stephen V.
Date : 17 January 2018
Identification Number : 10.1038/s41598-017-18575-5
Copyright Disclaimer : © The authors 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Uncontrolled Keywords : Bacteriology; Infectious-disease diagnostics; Pathogens
Depositing User : Clive Harris
Date Deposited : 19 Dec 2017 15:47
Last Modified : 14 Mar 2018 08:39
URI: http://epubs.surrey.ac.uk/id/eprint/845454

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