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Enterohaemorrhagic and other Shiga toxin producing Escherichia coli (STEC); where are we now regarding diagnostics and control strategies?

Newell, D and La Ragione, Roberto (2018) Enterohaemorrhagic and other Shiga toxin producing Escherichia coli (STEC); where are we now regarding diagnostics and control strategies? Transboundary and Emerging Diseases.

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Abstract

Escherichia coli comprises a highly diverse group of Gram-negative bacteria and is a common member of the intestinal microflora of humans and animals. Generally, such colonization is asymptomatic; however, some E. coli strains have evolved to become pathogenic and thus cause clinical disease in susceptible hosts. One pathotype, the Shiga toxigenic E. coli (STEC) comprising strains expressing a Shiga-like toxin is an important foodborne pathogen. A subset of STEC are the enterohaemorrhagic E. coli (EHEC), which can cause serious human disease, including haemolytic uraemic syndrome (HUS). The diagnosis of EHEC infections and the surveillance of STEC in the food chain and the environment require accurate, cost-effective and timely tests. In this review, we describe and evaluate tests now in routine use, as well as upcoming test technologies for pathogen detection, including loop-mediated isothermal amplification (LAMP) and whole-genome sequencing (WGS). We have considered the need for improved diagnostic tools in current strategies for the control and prevention of these pathogens in humans, the food chain and the environment. We conclude that although significant progress has been made, STEC still remains an important zoonotic issue worldwide. Substantial reductions in the public health burden due to this infection will require a multipronged approach, including ongoing surveillance with high-resolution diagnostic techniques currently being developed and integrated into the routine investigations of public health laboratories. However, additional research requirements may be needed before such high-resolution diagnostic tools can be used to enable the development of appropriate interventions, such as vaccines and decontamination strategies.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Newell, DUNSPECIFIEDUNSPECIFIED
La Ragione, RobertoR.Laragione@surrey.ac.ukUNSPECIFIED
Date : 25 January 2018
Identification Number : 10.1111/tbed.12789
Copyright Disclaimer : This is the peer reviewed version of the following article: Newell DG, La Ragione RM. Enterohaemorrhagic and other Shiga toxin-producing Escherichia coli (STEC): Where are we now regarding diagnostics and control strategies?. Transbound Emerg Dis. 2018;00:1–23. https://doi.org/10.1111/tbed.12789, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/tbed.12789/. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Depositing User : Melanie Hughes
Date Deposited : 06 Dec 2017 12:33
Last Modified : 31 Jan 2018 09:41
URI: http://epubs.surrey.ac.uk/id/eprint/845126

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