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Histopathological and immunohistochemical characterisation of Burkholderia pseudomallei lesions in an acute model of infection with BALB/c mice

García-Jiménez, Waldo L., Salguero Bodes, Francisco and D’Elia, Riccardo V. (2017) Histopathological and immunohistochemical characterisation of Burkholderia pseudomallei lesions in an acute model of infection with BALB/c mice International Journal of Experimental Pathology, 98 (6). pp. 347-355.

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Abstract

Organ tissue damage is a key contributor to host morbidity and mortality following infection with microbial agents. Severe immune responses, excessive cellular recruitment and necrosis of cells all play a role in disease pathology. Understanding the pathogenesis of disease can aid in identifying potential new therapeutic targets or simply act as a diagnostic tool. Burkholderia pseudomallei is a gram-negative bacterium that can cause acute and chronic diseases. The BALB/c mouse has been shown to be highly susceptible to aerosol challenge with B. pseudomallei and hence acts as a good model to study the acute and potentially lethal form of the disease melioidosis. In our study, BALB/c mice were challenged and culled at pre-determined time points to generate a pathological time course of infection. Lung, liver and spleen were subjected to pathological and immunohistochemical analysis. The number and type of microscopic lesions within each organ, as well as the location and the mean percentage of neutrophils, B cells, T cells and Burkholderia capsule antigen within the lesions were all characterised during the time course. Neutrophils were determined as the key player in tissue pathology and generation of lesions, with B cells playing an insignificant role. This detailed pathological assessment increases our understanding of B. pseudomallei disease.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
García-Jiménez, Waldo L.
Salguero Bodes, Franciscof.salguerobodes@surrey.ac.uk
D’Elia, Riccardo V.
Date : 27 February 2017
Identification Number : 10.1111/iep.12258
Copyright Disclaimer : © 2018 Crown copyright. International Journal of Experimental Pathology published by John Wiley & Sons Ltd on behalf of Company of the International Journal of Experimental Pathology (CIJEP). This article is published with the permission of the Controller of HMSO and the Queen’s Printer for Scotland. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : Burkholderia pseudomallei; Immunohistochemistry; Immunopathology; Neutrophils
Depositing User : Clive Harris
Date Deposited : 05 Dec 2017 15:44
Last Modified : 14 Mar 2018 13:15
URI: http://epubs.surrey.ac.uk/id/eprint/845117

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