University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Impact of liver fat on the differential partitioning of hepatic triacylglycerol into VLDL subclasses on high and low sugar diets

Umpleby, Margot, Shojaee-Moradie, Fariba, Fielding, Barbara, Liu, Xuefei, Marino, Andrea, Alsini, Najlaa, Isherwood, Cheryl, Jackson, Nicola, Ahmad, Aryati, Stolinski, Michael , Lovegrove, Julie A., Johnsen, Sigurd, Mendis, Agampodi, Wright, John, Wilinska, Malgorzata E., Hovorka, Roman, Bell, Jimmy D., Thomas, E. Louise, Frost, Gary S. and Griffin, Bruce (2017) Impact of liver fat on the differential partitioning of hepatic triacylglycerol into VLDL subclasses on high and low sugar diets Clinical Science, 131 (21). pp. 2561-2573.

[img] Text
Impact of liver fat on the differential partitioning of hepatic triacylglycerol into VLDL subclasses on high and low sugar diets.docx - Accepted version Manuscript
Restricted to Repository staff only until 10 November 2018.

Download (231kB)
[img] Text
Impact of liver fat on the differential partitioning of hepatic triacylglycerol into VLDL subclasses on high and low sugar diets - Supplementary Material 210417.docx - Supplemental Material
Restricted to Repository staff only until 10 November 2018.

Download (295kB)

Abstract

Dietary sugars are linked to the development of non-alcoholic fatty liver disease (NAFLD) and dyslipidaemia, but it is unknown if NAFLD itself influences the effects of sugars on plasma lipoproteins. To study this further, men with NAFLD (n=11) and low liver fat 'controls' (n= 14) were fed two iso-energetic diets, high or low in sugars (26% or 6% total energy) for 12 weeks, in a randomised, cross-over design. Fasting plasma lipid and lipoprotein kinetics were measured after each diet by stable isotope trace-labelling. There were significant differences in the production and catabolic rates of VLDL subclasses between men with NAFLD and controls, in response to the high and low sugar diets. Men with NAFLD had higher plasma concentrations of VLDL1-triacylglycerol (TAG) after the high ( P <0.02) and low sugar ( P <0.0002) diets, a lower VLDL1-TAG fractional catabolic rate after the high sugar diet ( P <0.01), and a higher VLDL1-TAG production rate after the low sugar diet ( P <0.01), relative to controls. An effect of the high sugar diet, was to channel hepatic TAG into a higher production of VLDL1-TAG ( P <0.02) in the controls, but in contrast, a higher production of VLDL2-TAG ( P <0.05) in NAFLD. These dietary effects on VLDL subclass kinetics could be explained, in part, by differences in the contribution of fatty acids from intra-hepatic stores, and de novo lipogenesis. This study provides new evidence that liver fat accumulation leads to a differential partitioning of hepatic TAG into large and small VLDL subclasses, in response to high and low intakes of sugars.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Umpleby, MargotM.Umpleby@surrey.ac.ukUNSPECIFIED
Shojaee-Moradie, FaribaUNSPECIFIEDUNSPECIFIED
Fielding, BarbaraB.Fielding@surrey.ac.ukUNSPECIFIED
Liu, XuefeiUNSPECIFIEDUNSPECIFIED
Marino, AndreaUNSPECIFIEDUNSPECIFIED
Alsini, NajlaaUNSPECIFIEDUNSPECIFIED
Isherwood, Cherylc.m.isherwood@surrey.ac.ukUNSPECIFIED
Jackson, NicolaUNSPECIFIEDUNSPECIFIED
Ahmad, AryatiUNSPECIFIEDUNSPECIFIED
Stolinski, MichaelUNSPECIFIEDUNSPECIFIED
Lovegrove, Julie A.UNSPECIFIEDUNSPECIFIED
Johnsen, SigurdS.Johnsen@surrey.ac.ukUNSPECIFIED
Mendis, Agampodia.mendis@surrey.ac.ukUNSPECIFIED
Wright, JohnJohn.Wright@surrey.ac.ukUNSPECIFIED
Wilinska, Malgorzata E.UNSPECIFIEDUNSPECIFIED
Hovorka, RomanUNSPECIFIEDUNSPECIFIED
Bell, Jimmy D.UNSPECIFIEDUNSPECIFIED
Thomas, E. LouiseUNSPECIFIEDUNSPECIFIED
Frost, Gary S.UNSPECIFIEDUNSPECIFIED
Griffin, BruceB.Griffin@surrey.ac.ukUNSPECIFIED
Date : 18 September 2017
Funders : Biological Biotechnology Scientific Research Council (BBSRC)
Identification Number : 10.1042/CS20171208
Copyright Disclaimer : © 2017 The Author(s)
Depositing User : Clive Harris
Date Deposited : 16 Nov 2017 11:51
Last Modified : 16 Nov 2017 11:54
URI: http://epubs.surrey.ac.uk/id/eprint/844935

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800