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The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles

Rotoli, Deborah, Cejas, Mariana-Mayela, Maeso, María-del-Carmen, Pérez-Rodríguez, Natalia-Dolores, Morales, Manuel, Ávila, Julio, Mobasheri, Ali and Martín-Vasallo, Pablo (2017) The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles International Journal of Molecular Sciences, 18 (11).

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Abstract

Glioblastoma multiforme (GBM) is the most common form of malignant glioma. Recent studies point out that gliomas exploit ion channels and transporters, including Na, K-ATPase, to sustain their singular growth and invasion as they invade the brain parenchyma. Moreover, the different isoforms of the β-subunit of Na, K-ATPase have been implicated in regulating cellular dynamics, particularly during cancer progression. The aim of this study was to determine the Na, K-ATPase β subunit isoform subcellular expression patterns in all cell types responsible for microenvironment heterogeneity of GBM using immunohistochemical analysis. All three isoforms, β1, β2/AMOG (Adhesion Molecule On Glia) and β3, were found to be expressed in GBM samples. Generally, β1 isoform was not expressed by astrocytes, in both primary and secondary GBM, although other cell types (endothelial cells, pericytes, telocytes, macrophages) did express this isoform. β2/AMOG and β3 positive expression was observed in the cytoplasm, membrane and nuclear envelope of astrocytes and GFAP (Glial Fibrillary Acidic Protein) negative cells. Interestingly, differences in isoforms expression have been observed between primary and secondary GBM: in secondary GBM, β2 isoform expression in astrocytes was lower than that observed in primary GBM, while the expression of the β3 subunit was more intense. These changes in β subunit isoforms expression in GBM could be related to a different ionic handling, to a different relationship between astrocyte and neuron (β2/AMOG) and to changes in the moonlighting roles of Na, K-ATPase β subunits as adaptor proteins and transcription factors.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Rotoli, DeborahUNSPECIFIEDUNSPECIFIED
Cejas, Mariana-MayelaUNSPECIFIEDUNSPECIFIED
Maeso, María-del-CarmenUNSPECIFIEDUNSPECIFIED
Pérez-Rodríguez, Natalia-DoloresUNSPECIFIEDUNSPECIFIED
Morales, ManuelUNSPECIFIEDUNSPECIFIED
Ávila, JulioUNSPECIFIEDUNSPECIFIED
Mobasheri, Alia.mobasheri@surrey.ac.ukUNSPECIFIED
Martín-Vasallo, PabloUNSPECIFIEDUNSPECIFIED
Date : 8 November 2017
Identification Number : 10.3390/ijms18112369
Copyright Disclaimer : © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords : Glioblastoma multiforme; Na, K-ATPase; Sodium pump; Na, K-ATPase β subunit isoforms; Moonlighting proteins; β2/AMOG; Glioblastoma multiforme microenvironment; Astrocyte-neuron adhesion; Two-Hybrid system
Depositing User : Clive Harris
Date Deposited : 14 Nov 2017 16:27
Last Modified : 14 Nov 2017 16:27
URI: http://epubs.surrey.ac.uk/id/eprint/844914

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