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Experimental Work Towards the Improvement of a Kinetic Model for Acetone-Butanol-Ethanol Pathway

Rahimsalehi, A, Avignone-Rossa, C and Arellano-Garcia, Harvey (2017) Experimental Work Towards the Improvement of a Kinetic Model for Acetone-Butanol-Ethanol Pathway In: 27th European Symposium on Computer Aided Process Engineering – ESCAPE 27, Oct 1 - 5 2017, Barcelona, Spain.

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Abstract

In recent years, the production of waste materials because of population growth is increasing. Accordingly, finding new methods to convert such materials into fuel are becoming more popular. The objective of this study is to determine the potential of dried distiller`s grains and soluble (DDGS), a by-product of bioethanol industry, to produce butanol using strains of Clostridium saccharoperbutylacetonicum (C.SCC). In addition, this research is intended to develop the fermentation kinetic by improving the bacterial growth and medium optimisation while enhancing the process parameters based on the experimental data. Therefore, to find the most optimum condition for DDGS as the growth medium, the crucial effect of pH and various DDGS media supplementation were identified. Consequently, after performing several experiments in batch serum bottles without agitation, it had been determined that the best condition for DDGS as the growth medium is supplementing the autoclaved DDGS medium with 10 % (v/v) sucrose solution while pH is adjusted to 6.5. Thus, when the supplemented DDGS medium was inoculated with 10 % (v/v) of Clostridium saccharoperbutylacetonicum, the butanol concentration improved to approximately 7.2 (g/L) while this amount for the non-supplemented DDGS medium was approximately 4.4 (g/L). However, under the same conditions for DDGS as the growth medium, the butanol concentration decrease to 0.22 (g/L) while agitation was involved in the batch bioreactor. Based on the experimental results obtained from various experiments of this research, it is concluded that Clostridium saccharoperbutylacetonicum microorganism can ferment sucrose and other carbon sources available in DDGS such as glucose. Moreover, to improve the butanol concentration using mathematical modeling and computer simulation in ongoing studies, the collected preliminary data of this experimental research could be used in the proposed kinetic model by Shinto et al. (2007).

Item Type: Conference or Workshop Item (Conference Paper)
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
NameEmailORCID
Rahimsalehi, A
Avignone-Rossa, C
Arellano-Garcia, Harveyh.arellano-garcia@surrey.ac.uk
Date : 9 November 2017
Identification Number : 10.1016/B978-0-444-63965-3.50481-5
Copyright Disclaimer : © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Acetone-Butanol-Ethanol Fermentation; Biofuel production; Kinetic model; Clostridium saccharoperbutylacetonicum N1-4;
Related URLs :
Depositing User : Melanie Hughes
Date Deposited : 22 Sep 2017 16:42
Last Modified : 17 Jan 2018 14:36
URI: http://epubs.surrey.ac.uk/id/eprint/842393

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