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Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens

Riitho, Victor, Walters, Adam A., Somavarapu, Satyanarayana, Lamp, Benjamin, Rümenapf, Till, Krey, Thomas, Rey, Felix A., Oviedo-Orta, Ernesto, Stewart, Graham R., Locker, Nicolas , Steinbach, Falko and Graham, Simon (2017) Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens Scientific Reports, 7, 13743.

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Abstract

Subunit viral vaccines are typically not as efficient as live attenuated or inactivated vaccines at inducing protective immune responses. This paper describes an alternative ‘biomimetic’ technology; whereby viral antigens were formulated around a polymeric shell in a rationally arranged fashion with a surface glycoprotein coated on to the surface and non-structural antigen and adjuvant encapsulated. We evaluated this model using BVDV E2 and NS3 proteins formulated in poly-(D, L-lactic-co-glycolic acid) (PLGA) nanoparticles adjuvanted with polyinosinic:polycytidylic acid (poly(I:C) as an adjuvant (Vaccine-NP). This Vaccine-NP was compared to ovalbumin and poly(I:C) formulated in a similar manner (Control-NP) and a commercial adjuvanted inactivated BVDV vaccine (IAV), all inoculated subcutaneously and boosted prior to BVDV-1 challenge. Significant virus-neutralizing activity, and E2 and NS3 specific antibodies were observed in both Vaccine-NP and IAV groups following the booster immunisation. IFN-γ responses were observed in ex vivo PBMC stimulated with E2 and NS3 proteins in both vaccinated groups. We observed that the protection afforded by the particulate vaccine was comparable to the licenced IAV formulation. In conclusion, the biomimetic particulates showed a promising immunogenicity and efficacy profile that may be improved by virtue of being a customisable mode of delivery.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Riitho, VictorUNSPECIFIEDUNSPECIFIED
Walters, Adam A.UNSPECIFIEDUNSPECIFIED
Somavarapu, SatyanarayanaUNSPECIFIEDUNSPECIFIED
Lamp, BenjaminUNSPECIFIEDUNSPECIFIED
Rümenapf, TillUNSPECIFIEDUNSPECIFIED
Krey, ThomasUNSPECIFIEDUNSPECIFIED
Rey, Felix A.UNSPECIFIEDUNSPECIFIED
Oviedo-Orta, ErnestoUNSPECIFIEDUNSPECIFIED
Stewart, Graham R.UNSPECIFIEDUNSPECIFIED
Locker, NicolasN.Locker@surrey.ac.ukUNSPECIFIED
Steinbach, Falkof.steinbach@surrey.ac.ukUNSPECIFIED
Graham, Simons.graham@surrey.ac.ukUNSPECIFIED
Date : 2017
Identification Number : 10.1038/s41598-017-13915-x
Copyright Disclaimer : © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Depositing User : Clive Harris
Date Deposited : 21 Sep 2017 08:23
Last Modified : 26 Oct 2017 13:45
URI: http://epubs.surrey.ac.uk/id/eprint/842361

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