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Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

Loizides-Mangold, U, Perrin, L, Vandereycken, B, Betts, JA, Walhin, J-P, Templeman, I, Chanon, S, Weger, BD, Durand, C, Robert, M , Paz, J, Moniatte, M, Karagounis, LG, Johnston, Jonathan, Gachon, F, Lefai, E, Riezman, H and Dibner, C (2017) Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro PNAS.

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Official URL: http://www.pnas.org/

Abstract

Circadian clocks play an important role in lipid homeostasis with impact on various metabolic diseases. Due to the central role of skeletal muscle in whole-body metabolism we aimed at studying muscle lipid profiles in a temporal manner. Moreover, it has not been shown whether lipid oscillations in peripheral tissues are driven by diurnal cycles of rest/activity and food intake or are able to persist in vitro in a cell autonomous manner. To address this, we investigated lipid profiles over 24 h in human skeletal muscle in vivo, and in primary human myotubes cultured in vitro. Glycerolipids, glycerophospholipids and sphingolipids exhibited diurnal oscillations, suggesting a widespread circadian impact on muscle lipid metabolism. Notably, peak levels of lipid accumulation were in phase coherence with core clock gene expression in vivo and in vitro. The percentage of oscillating lipid metabolites was comparable between muscle tissue and cultured myotubes, and temporal lipid profiles correlated with transcript profiles of genes implicated in their biosynthesis. Lipids enriched in the outer leaflet of the plasma membrane oscillated in a highly coordinated manner in vivo and in vitro. Lipid metabolite oscillations were strongly attenuated upon siRNA-mediated clock disruption in human primary myotubes. Taken together, our data suggest an essential role for endogenous cell-autonomous human skeletal muscle oscillators in regulating lipid metabolism, independent of external synchronizers such as physical activity or food intake.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Loizides-Mangold, UUNSPECIFIEDUNSPECIFIED
Perrin, LUNSPECIFIEDUNSPECIFIED
Vandereycken, BUNSPECIFIEDUNSPECIFIED
Betts, JAUNSPECIFIEDUNSPECIFIED
Walhin, J-PUNSPECIFIEDUNSPECIFIED
Templeman, IUNSPECIFIEDUNSPECIFIED
Chanon, SUNSPECIFIEDUNSPECIFIED
Weger, BDUNSPECIFIEDUNSPECIFIED
Durand, CUNSPECIFIEDUNSPECIFIED
Robert, MUNSPECIFIEDUNSPECIFIED
Paz, JUNSPECIFIEDUNSPECIFIED
Moniatte, MUNSPECIFIEDUNSPECIFIED
Karagounis, LGUNSPECIFIEDUNSPECIFIED
Johnston, JonathanJ.Johnston@surrey.ac.ukUNSPECIFIED
Gachon, FUNSPECIFIEDUNSPECIFIED
Lefai, EUNSPECIFIEDUNSPECIFIED
Riezman, HUNSPECIFIEDUNSPECIFIED
Dibner, CUNSPECIFIEDUNSPECIFIED
Date : 25 September 2017
Identification Number : 10.1073/pnas.1705821114
Copyright Disclaimer : Copyright 2017 The Author(s). Published by National Academy of Sciences.
Depositing User : Melanie Hughes
Date Deposited : 26 Sep 2017 10:33
Last Modified : 26 Sep 2017 10:53
URI: http://epubs.surrey.ac.uk/id/eprint/842315

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