Rapid, secure drug testing using fingerprint development and paper spray mass spectrometry

Costa, Catia, Webb, Roger, Palitsin, Vladimir, Ismail, Mahado, de Puit, Marcel, Atkinson, Samuel and Bailey, Melanie (2017) Rapid, secure drug testing using fingerprint development and paper spray mass spectrometry Clinical Chemistry, 63 (11). pp. 1745-1752.

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Abstract

BACKGROUND: Paper spray mass spectrometry6 is a technique that has recently emerged and has shown excellent analytical sensitivity to a number of drugs in blood. As an alternative to blood, fingerprints have been shown to provide a noninvasive and traceable sampling matrix. Our goal was to validate the use of fingerprint samples to detect cocaine use.

METHODS: Samples were collected on triangular pieces (168 mm2) of washed Whatman Grade I chromatography paper. Following application of internal standard, spray solvent and a voltage were applied to the paper before mass spectrometry detection. A fingerprint visualization step was incorporated into the analysis procedure by addition of silver nitrate solution and exposing the sample to ultraviolet light.

RESULTS: Limits of detection for cocaine, benzoylecgonine, and methylecgonine were 1, 2, and 31 ng/mL respectively, with relative standard deviations of less than 33%. No matrix effects were observed. Analysis of 239 fingerprint samples yielded a 99% true-positive rate and a 2.5% false-positive rate, based on the detection of cocaine, benzoylecgonine, or methylecgonine with use of a single fingerprint.

CONCLUSIONS: The method offers a qualitative and noninvasive screening test for cocaine use. The analysis method developed is rapid (4 min/sample) and requires no sample preparation.

Item Type:	Article
Divisions :	Faculty of Engineering and Physical Sciences > Electronic Engineering
Authors :	Name Email ORCID Costa, Catia cc00192@surrey.ac.uk Webb, Roger R.Webb@surrey.ac.uk Palitsin, Vladimir V.Palitsin@surrey.ac.uk Ismail, Mahado mahado.ismail@surrey.ac.uk de Puit, Marcel Atkinson, Samuel Bailey, Melanie M.Bailey@surrey.ac.uk
Date :	22 September 2017
DOI :	10.1373/clinchem.2017.275578
Copyright Disclaimer :	© 2017 American Association for Clinical Chemistry
Depositing User :	Clive Harris
Date Deposited :	15 Sep 2017 11:54
Last Modified :	07 Feb 2019 16:39
URI:	http://epubs.surrey.ac.uk/id/eprint/842287

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