University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus

Strandbygaard, Bertel, Lavazza, Antonio, Lelli, Davide, Blanchard, Yannick, Grasland, Béatrice, Poder, Sophie Le, Rose, Nicolas, Steinbach, Falko, van der Poel, Wim H.M., Widén, Frederik , Belsham, Graham J. and Bøtner, Anette (2016) Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus Veterinary Microbiology, 197. pp. 151-160.

[img] Text
StrandbygaardetalPEDserologyrevised_accepted.docx - Accepted version Manuscript
Restricted to Repository staff only until 21 November 2017.

Download (58kB)
[img] Text
StrandbygaardetalTable1A.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (19kB)
[img] Text
StrandbygaardetalTable1B.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (18kB)
[img] Text
StrandbygaardetalTable2.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (14kB)
[img] Text
StrandbygaardetalTable3.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (15kB)
[img] Text
StrandbygaardetalTable4.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (16kB)
[img] Text
StrandbygaardetalTable5.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (15kB)
[img] Text
StrandbygaardetalTable6AB.docx - Supplemental Material
Restricted to Repository staff only until 21 November 2017.

Download (13kB)

Abstract

Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of “in house” ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Strandbygaard, BertelUNSPECIFIEDUNSPECIFIED
Lavazza, AntonioUNSPECIFIEDUNSPECIFIED
Lelli, DavideUNSPECIFIEDUNSPECIFIED
Blanchard, YannickUNSPECIFIEDUNSPECIFIED
Grasland, BéatriceUNSPECIFIEDUNSPECIFIED
Poder, Sophie LeUNSPECIFIEDUNSPECIFIED
Rose, NicolasUNSPECIFIEDUNSPECIFIED
Steinbach, Falkof.steinbach@surrey.ac.ukUNSPECIFIED
van der Poel, Wim H.M.UNSPECIFIEDUNSPECIFIED
Widén, FrederikUNSPECIFIEDUNSPECIFIED
Belsham, Graham J.UNSPECIFIEDUNSPECIFIED
Bøtner, AnetteUNSPECIFIEDUNSPECIFIED
Date : 2016
Identification Number : 10.1016/j.vetmic.2016.11.020
Uncontrolled Keywords : ELISA; Porcine coronavirus; PEDV; Ring trial; Serology
Depositing User : Clive Harris
Date Deposited : 08 Sep 2017 09:20
Last Modified : 08 Sep 2017 09:20
URI: http://epubs.surrey.ac.uk/id/eprint/842214

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800