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Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus

Strandbygaard, Bertel, Lavazza, Antonio, Lelli, Davide, Blanchard, Yannick, Grasland, Béatrice, Poder, Sophie Le, Rose, Nicolas, Steinbach, Falko, van der Poel, Wim H.M., Widén, Frederik , Belsham, Graham J. and Bøtner, Anette (2016) Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus Veterinary Microbiology, 197. pp. 151-160.

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Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of “in house” ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
Strandbygaard, Bertel
Lavazza, Antonio
Lelli, Davide
Blanchard, Yannick
Grasland, Béatrice
Poder, Sophie Le
Rose, Nicolas
van der Poel, Wim H.M.
Widén, Frederik
Belsham, Graham J.
Bøtner, Anette
Date : 2016
DOI : 10.1016/j.vetmic.2016.11.020
Copyright Disclaimer : © 2016 Published by Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Uncontrolled Keywords : ELISA; Porcine coronavirus; PEDV; Ring trial; Serology
Depositing User : Clive Harris
Date Deposited : 08 Sep 2017 09:20
Last Modified : 16 Jan 2019 18:56

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