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Generation of ribosome imprinted polymers for sensitive detection of translational responses

King, Helen A., El-Sharif, Hazim F., Matia-González, Ana M., Iadevaia, Valentina, Fowotade, Adeola, Reddy, Subrayal M. and Gerber, Andre (2017) Generation of ribosome imprinted polymers for sensitive detection of translational responses Scientific Reports, 7 (1).

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Abstract

Whilst the profiling of the transcriptome and proteome even of single-cells becomes feasible, the analysis of the translatome, which refers to all messenger RNAs (mRNAs) engaged with ribosomes for protein synthesis, is still an elaborate procedure requiring millions of cells. Herein, we report the generation and use of “smart materials”, namely molecularly imprinted polymers (MIPs) to facilitate the isolation of ribosomes and translated mRNAs from merely 1,000 cells. In particular, we show that a hydrogel-based ribosome imprinted polymer could recover ribosomes and associated mRNAs from human, simian and mice cellular extracts, but did not selectively enrich yeast ribosomes, thereby demonstrating selectivity. Furthermore, ribosome imprinted polymers enabled the sensitive measurement of an mRNA translational regulatory event, requiring 1,000-fold less cells than current methodologies. These results provide first evidence for the suitability of MIPs to selectively recover ribonucleoprotein complexes such as ribosomes, founding a novel means for sensitive detection of gene regulation.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
King, Helen A.UNSPECIFIEDUNSPECIFIED
El-Sharif, Hazim F.UNSPECIFIEDUNSPECIFIED
Matia-González, Ana M.UNSPECIFIEDUNSPECIFIED
Iadevaia, Valentinav.iadevaia@surrey.ac.ukUNSPECIFIED
Fowotade, AdeolaUNSPECIFIEDUNSPECIFIED
Reddy, Subrayal M.UNSPECIFIEDUNSPECIFIED
Gerber, Andrea.gerber@surrey.ac.ukUNSPECIFIED
Date : 26 July 2017
Identification Number : 10.1038/s41598-017-06970-x
Copyright Disclaimer : © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Depositing User : Clive Harris
Date Deposited : 14 Aug 2017 07:35
Last Modified : 14 Aug 2017 07:35
URI: http://epubs.surrey.ac.uk/id/eprint/841896

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