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Identification of FadAB complexes involved in fatty acid ß-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing strain.

Menendez-Bravo, S, Paganini, J, Avignone Rossa, Claudio, Gramajo, H and Arabolaza, A (2017) Identification of FadAB complexes involved in fatty acid ß-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing strain. Frontiers in Microbiology, 8, 1428.

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Abstract

Oleaginous microorganisms represent possible platforms for the sustainable production of oleochemicals and biofuels due to their metabolic robustness and the possibility to be engineered. Streptomyces coelicolor is among the narrow group of prokaryotes capable of accumulating triacylglycerol (TAG) as carbon and energy reserve. Although the pathways for TAG biosynthesis in this organism have been widely addressed, the set of genes required for their breakdown have remained elusive so far. Here, we identified and characterized three gene clusters involved in the β‐oxidation of fatty acids (FA). The role of each of the three different S. coelicolor FadAB proteins in fatty acid catabolism was confirmed by complementation of an E. coliΔfadBA mutant strain deficient in β‐oxidation. In S. coelicolor, the expression profile of the three gene clusters showed variation related with the stage of growth and the presence of FA in media. Flux balance analyses using a corrected version of the current S. coelicolor metabolic model containing detailed TAG biosynthesis reactions suggested the relevance of the identified fadAB genes in the accumulation of TAG. Thus, through the construction and analysis of fadAB knock out mutant strains, we obtained an S. coelicolor mutant that showed a 4.3-fold increase in the TAG content compared to the wild type strain grown under the same culture conditions.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Menendez-Bravo, SUNSPECIFIEDUNSPECIFIED
Paganini, JUNSPECIFIEDUNSPECIFIED
Avignone Rossa, ClaudioC.Avignone-Rossa@surrey.ac.ukUNSPECIFIED
Gramajo, HUNSPECIFIEDUNSPECIFIED
Arabolaza, AUNSPECIFIEDUNSPECIFIED
Date : 2 August 2017
Funders : BBSRC
Identification Number : 10.3389/fmicb.2017.01428
Copyright Disclaimer : Copyright © 2017 Menendez-Bravo, Paganini, Avignone-Rossa, Gramajo and Arabolaza. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords : Streptomyces coelicolor, Triacylglycerol accumulation, Beta-oxidation of fatty acids, Neutral lipid, chemostat system
Related URLs :
Depositing User : Melanie Hughes
Date Deposited : 19 Jul 2017 15:19
Last Modified : 29 Sep 2017 16:50
URI: http://epubs.surrey.ac.uk/id/eprint/841710

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