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Microglia in Culture: What Genes Do They Express?

Duke, Dawn, Moran, L.B, Turkheimer, F.E, Banati, R and Graeber, M.B (2004) Microglia in Culture: What Genes Do They Express? Developmental Neuroscience, 26 (1). pp. 30-37.

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Abstract

The cell culture model utilized in this study represents one of the most widely used paradigms of microglia in vitro. After 14 days, microglia harvested from the neonatal rat brain are considered ‘mature’. However, it is clear that this represents a somewhat arbitrary definition. In this paper, we provide a transcriptome definition of such microglial cells. More than 7,000 known genes and 1,000 expressed sequence tag clusters were analysed. ‘Microglia genes’ were defined as sequences consistently expressed in all microglia samples tested. Accordingly, 388 genes were identified as microglia genes. Another 1,440 sequences were detected in a subset of the cultures. Genes consistently expressed by microglia included genes known to be involved in the cellular immune response, brain tissue surveillance, microglial migration as well as proliferation. The expression profile reported here provides a baseline against which changes of microglia in vitro can be examined. Importantly, expression profiling of normal microglia will help to provide the presently purely operational definition of ‘microglial activation’ with a molecular biological correlate. Furthermore, the data reported here add to our understanding of microglia biology and allow projections as to what functions microglia may exert in vivo, as well as in vitro.

Item Type: Article
Divisions : Researcher Development Programme
Authors :
NameEmailORCID
Duke, DawnD.Duke@surrey.ac.ukUNSPECIFIED
Moran, L.BUNSPECIFIEDUNSPECIFIED
Turkheimer, F.EUNSPECIFIEDUNSPECIFIED
Banati, RUNSPECIFIEDUNSPECIFIED
Graeber, M.BUNSPECIFIEDUNSPECIFIED
Date : 22 October 2004
Copyright Disclaimer : Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center. © 2004 S. Karger AG, Basel
Uncontrolled Keywords : Brain macrophages; Cell type-specific gene expression signature; Microglia cultures; Microglial sensor of pathology; Transcriptomics
Depositing User : Jane Hindle
Date Deposited : 22 Jun 2017 15:28
Last Modified : 22 Jun 2017 15:28
URI: http://epubs.surrey.ac.uk/id/eprint/841468

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