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The microglial gene regulatory network activated by interferon-gamma

Moran, Linda B, Duke, Dawn and Graeber, Manuel B (2006) The microglial gene regulatory network activated by interferon-gamma Journal of Neuroimmunology, 183 (1–2). pp. 1-6.

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We have analysed the microglial pathway stimulated by interferon-gamma (IFN-gamma) using an in silico approach employing a database of eukaryotic molecular interactions and a microarray dataset validated by quantitative real-time PCR (qRT-PCR). Following IFN-gamma stimulation, production of neuroprotective factors by microglia was found to be reduced while caspase 1 and serping1 which are involved in cell death cascades are up-regulated suggesting a safeguarding mechanism. Extracellular matrix interactions and intracellular protein degradation are altered in concert with these changes. The regulatory network of IFN-gamma responsive microglial genes is outlined in detail and differentially expressed genes are mapped to their respective cellular compartments. A pathway approach to the analysis of microarray data is advocated since overlaying pathway and actual expression data as shown here greatly facilitates understanding the biological meaning of a gene regulatory network. In addition, genes of similar function that are differentially regulated are less likely to be false positives than single unrelated genes.

Item Type: Article
Divisions : Researcher Development Programme
Authors :
Moran, Linda B
Graeber, Manuel B
Date : 22 December 2006
OA Location : 10.1016/j.jneuroim.2006.10.023
Copyright Disclaimer : Copyright © 2006 Elsevier B.V. All rights reserved.
Uncontrolled Keywords : Antigen presentation Expression profiling Microarrays; Microglial function Pathway signature Pathway signature
Depositing User : Jane Hindle
Date Deposited : 22 Jun 2017 12:29
Last Modified : 16 Jan 2019 18:53

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