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Delayed persistence of giant-nucleated cells induced by X-ray and proton irradiation in the progeny of replicating normal human fibroblast cells

Almahwasi, A.A, Jeynes, J.C., Merchant, M.J., Bradley, D.A. and Regan, P.H. (2016) Delayed persistence of giant-nucleated cells induced by X-ray and proton irradiation in the progeny of replicating normal human fibroblast cells Radiation Physics and Chemistry, 137. pp. 163-168.

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Abstract

Ionising radiation can induce giant-nucleated cells (GCs) in the progeny of irradiated populations, as demonstrated in various cellular systems. Most in vitro studies have utilised quiescent cancerous or normal cell lines but it is not clear whether radiation-induced GCs persist in the progeny of normal replicated cells. In the current work we show persistent induction of GCs in the progeny of normal human-diploid skin fibroblasts (AG1522). These cells were originally irradiated with a single equivalent clinical dose of 0.2, 1 or 2 Gy of either X-ray or proton irradiation and maintained in an active state for various post-irradiation incubation interval times before they were replated for GC analysis. The results demonstrate that the formation of GCs in the progeny of X-ray or proton irradiated cells was increased in a dose-dependent manner when measured 7 days after irradiation and this finding is in agreement with that reported for the AG1522 cells using other radiation qualities. For the 1 Gy X-ray doses it was found that the GC yield increased continually with time up to 21 days post-irradiation. These results can act as benchmark data for such work and may have important implications for studies aimed at evaluating the efficacy of radiation therapy and in determining the risk of delayed effects particularly when applying protons.

Item Type: Article
Authors :
NameEmailORCID
Almahwasi, A.AUNSPECIFIEDUNSPECIFIED
Jeynes, J.C.UNSPECIFIEDUNSPECIFIED
Merchant, M.J.UNSPECIFIEDUNSPECIFIED
Bradley, D.A.UNSPECIFIEDUNSPECIFIED
Regan, P.H.UNSPECIFIEDUNSPECIFIED
Date : 2 March 2016
Identification Number : https://doi.org/10.1016/j.radphyschem.2016.02.030
Copyright Disclaimer : Copyright 2016 Elsevier Ltd.All rights reserved.
Additional Information : Full text not available from this repository
Depositing User : Melanie Hughes
Date Deposited : 13 Jun 2017 09:16
Last Modified : 13 Jun 2017 09:46
URI: http://epubs.surrey.ac.uk/id/eprint/841352

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