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Sonodynamic therapy combined with novel anti-cancer agents, sanguinarine and ginger root extract: Synergistic increase in toxicity in the presence of PANC-1 cells in vitro.

Prescott, M, Mitchell, J, Totti, Styliani, Lee, Judy, Velliou, Eirini and Bussemaker, Madeleine (2017) Sonodynamic therapy combined with novel anti-cancer agents, sanguinarine and ginger root extract: Synergistic increase in toxicity in the presence of PANC-1 cells in vitro. Ultrasonics Sonochemistry.

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Abstract

The presence of ultrasound-induced cavitation in sonodynamic therapy (SDT) treatments has previously enhanced the activity and delivery of certain sonosensitisers in biological systems. The purpose of this work was to investigate the potential for two novel anti-cancer agents from natural derivatives, sanguinarine and ginger root extract (GRE), as sonosensitisers in an SDT treatment with in vitro PANC-1 cells. Both anti-cancer compounds had a dose-dependent cytotoxicity in the presence of PANC-1 cells. A range of six discreet ultrasound power-frequency configurations were tested and it was found that the cell death caused directly by ultrasound was likely due to the sonomechanical effects of cavitation. Combined treatment used dosages of 100 μM sanguinarine or 1mM of GRE with 15 s sonication at 500 kHz and 10 W. The sanguinarine-SDT and GRE-SDT treatments showed a 6% and 17% synergistic increase in observed cell death, respectively. Therefore both sangunarine and GRE were found to be effective sonosensitisers and warrant further development for SDT, with a view to maximising the magnitude of synergistic increase in toxicity.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
NameEmailORCID
Prescott, MUNSPECIFIEDUNSPECIFIED
Mitchell, JUNSPECIFIEDUNSPECIFIED
Totti, Stylianis.totti@surrey.ac.ukUNSPECIFIED
Lee, Judyj.y.lee@surrey.ac.ukUNSPECIFIED
Velliou, Eirinie.velliou@surrey.ac.ukUNSPECIFIED
Bussemaker, Madeleinem.bussemaker@surrey.ac.ukUNSPECIFIED
Date : 13 May 2017
Identification Number : https://doi.org/10.1016/j.ultsonch.2017.05.018
Copyright Disclaimer : © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Sonodynamic therapy, pancreatic cancer, sonosensitiser, sanguinarine, ginger root extract.
Depositing User : Melanie Hughes
Date Deposited : 24 May 2017 13:29
Last Modified : 24 May 2017 15:40
URI: http://epubs.surrey.ac.uk/id/eprint/841129

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