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Towards the quantification of the effect of acid treatment on the heat tolerance of Escherichia coli K12 at lethal temperatures.

Velliou, EG, Van Derlinden, E, Cappuyns, AM, Nikolaidou, E, Geeraerd, AH, Devlieghere, F and Van Impe, JF (2011) Towards the quantification of the effect of acid treatment on the heat tolerance of Escherichia coli K12 at lethal temperatures. Food Microbiol, 28 (4). pp. 702-711.

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Abstract

The aim of this work is to investigate the effect of acid treatment -before and during heat inactivation- on the heat resistance of Escherichia coli K12 MG1655 cells at lethal temperatures. E. coli cells were grown in Brain Heart Infusion broth until they reached the stationary phase (≈10(9) cfu/mL). Approximately 30 min before thermal inactivation the early stationary phase cells were added in Brain Heart Infusion broth with a specific pH value, achieved with addition of either acetic (50% (v/v)), lactic (50% (v/v)) or hydrochloric acid (30% (v/v)), and inactivation experiments took place at 54 °C and 58 °C. The inactivation dynamics are analysed using the inactivation model of Geeraerd et al. (2000). This enables to define the induced thermotolerance of E. coli as a prolongation of the shoulder and/or a reduction of the inactivation rate. Generally, addition of acids increased the heat resistance of E. coli. The induced resistance depends on the type of acid and on the quantity added, i.e. different levels of acidification lead to a different level of heat resistance. This work provides additional knowledge on the reaction of bacterial cultures to heat after acid treatment -before and during heat treatment- and, therefore, it contributes to an improved understanding of the effect of acid exposure on the bacterial heat resistance.

Item Type: Article
Authors :
NameEmailORCID
Velliou, EGe.velliou@surrey.ac.ukUNSPECIFIED
Van Derlinden, EUNSPECIFIEDUNSPECIFIED
Cappuyns, AMUNSPECIFIEDUNSPECIFIED
Nikolaidou, EUNSPECIFIEDUNSPECIFIED
Geeraerd, AHUNSPECIFIEDUNSPECIFIED
Devlieghere, FUNSPECIFIEDUNSPECIFIED
Van Impe, JFUNSPECIFIEDUNSPECIFIED
Date : June 2011
Identification Number : https://doi.org/10.1016/j.fm.2010.06.007
Uncontrolled Keywords : Adaptation, Physiological, Colony Count, Microbial, Escherichia coli K12, Hot Temperature, Hydrogen-Ion Concentration, Models, Biological
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 13:23
Last Modified : 17 May 2017 15:10
URI: http://epubs.surrey.ac.uk/id/eprint/839001

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