University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Phytochemical and pharmacological screening of Sterculiaceae species and isolation of antibacterial compounds.

Reid, KA, Jäger, AK, Light, ME, Mulholland, DA and Van Staden, J (2005) Phytochemical and pharmacological screening of Sterculiaceae species and isolation of antibacterial compounds. J Ethnopharmacol, 97 (2). pp. 285-291.

Full text not available from this repository.

Abstract

Little previous phytochemical investigation has been conducted on South African Sterculiaceae species used in traditional medicine. In this study, five species, varying in growth type (small herbs, shrubs and large trees) and traditional usage were investigated. The species screened were Cola greenwayi Brenan, Cola natalensis Oliv., Dombeya burgessiae Gerr. ex Harv., Dombeya cymosa Harv. and Hermannia depressa N.E.Br. Extracts were screened for alkaloids, cardiac glycosides, cyanogenic glycosides, saponins and tannins. The probable presence of bufadienolides in the leaf material of Dombeya burgessiae and Dombeya cymosa was determined. Alkaloids, cyanogenic glycosides and saponins were absent in all the plant material investigated. Tannins were detected in the leaf extract of Cola greenwayi and in the leaves, stems and roots of Hermannia depressa. Extracts were screened for anti-inflammatory and antibacterial activity using the cyclooxygenase-1 (COX-1) inhibition assay and the microdilution antibacterial assay. The ethanol and dichloromethane extracts of Cola greenwayi, Dombeya burgessiae and Dombeya cymosa, and the dichloromethane extracts of Hermannia depressa showed the highest levels of COX-1 inhibition. It is possible that the high levels observed may be due to the presence of tannins in some of the extracts. Generally, all the aqueous extracts exhibited low activity. Similarly, no antibacterial activity was observed with the aqueous extracts, although some mild activity was exhibited with some of the ethanol and ethyl acetate extracts. Following the general phytochemical and pharmacological screening, extracts showing antibacterial activity were further purified using bioassay-guided fractionation. Dombeya rotundifolia (Hochst.) Planch., which was screened in a previous study, was also included in the isolation of active compounds. A bioautographic assay, using Staphylococcus aureus, was used to detect the presence of the antibacterial compounds. These were isolated and identified as fatty acids.

Item Type: Article
Authors :
NameEmailORCID
Reid, KAUNSPECIFIEDUNSPECIFIED
Jäger, AKUNSPECIFIEDUNSPECIFIED
Light, MEUNSPECIFIEDUNSPECIFIED
Mulholland, DAd.mulholland@surrey.ac.ukUNSPECIFIED
Van Staden, JUNSPECIFIEDUNSPECIFIED
Date : 28 February 2005
Identification Number : 10.1016/j.jep.2004.11.010
Uncontrolled Keywords : Anti-Bacterial Agents, Anti-Inflammatory Agents, Cyclooxygenase 1, Cyclooxygenase Inhibitors, Medicine, African Traditional, Microbial Sensitivity Tests, Plant Extracts, Plant Leaves, Prostaglandin-Endoperoxide Synthases, South Africa, Staphylococcus aureus, Sterculiaceae
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 12:55
Last Modified : 17 May 2017 15:06
URI: http://epubs.surrey.ac.uk/id/eprint/837246

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800