Dielectrophoresis of poly at and poly GC DNA Nanomanipulation

Mohamad, AS, Jeynes, JC and Hughes, MP (2012) Dielectrophoresis of poly at and poly GC DNA Nanomanipulation

Full text not available from this repository.

Abstract

Dielectrophoresis (DEP) is the induced motion of polarizable particles in non-uniform electric fields. Used for many years for the manipulation of particles from cell-scale to macromolecules, we present here the application of the technique for manipulation of DNA containing only adenine-thymine (poly AT) bases, and that of DNA containing only cytosine-guanine (poly GC), using microfabricated electrode structures. Poly AT was stained with DAPI and JOJO-1 for poly GC DNA. It was found that there were differences between the frequency-dependent DEP behavior of the two molecules; when looking at the difference between the two types on crossover frequency (the point where DEP changes from attractive to repulsive), it was found that they varied by up to a factor of 2. This points to possible insigns in the charge conduction mechanism in different DNA forms, as well as potential new mechanisms for gene separations and sequencing. © 2012 IEEE.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Divisions :	Surrey research (other units)
Authors :	Name Email ORCID Mohamad, AS Jeynes, JC j.c.jeynes@surrey.ac.uk Hughes, MP m.hughes@surrey.ac.uk
Date :	22 November 2012
DOI :	10.1109/NANO.2012.6321928
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 12:38
Last Modified :	23 Jan 2020 17:59
URI:	http://epubs.surrey.ac.uk/id/eprint/836110

Actions (login required)

View Item

Downloads