Dielectrophoresis of poly at and poly GC DNA Nanomanipulation

Mohamad, AS, Jeynes, JC and Hughes, MP (2012) Dielectrophoresis of poly at and poly GC DNA Nanomanipulation

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Abstract

Dielectrophoresis (DEP) is the induced motion of polarizable particles in non-uniform electric fields. Used for many years for the manipulation of particles from cell-scale to macromolecules, we present here the application of the technique for manipulation of DNA containing only adenine-thymine (poly AT) bases, and that of DNA containing only cytosine-guanine (poly GC), using microfabricated electrode structures. Poly AT was stained with DAPI and JOJO-1 for poly GC DNA. It was found that there were differences between the frequency-dependent DEP behavior of the two molecules; when looking at the difference between the two types on crossover frequency (the point where DEP changes from attractive to repulsive), it was found that they varied by up to a factor of 2. This points to possible insigns in the charge conduction mechanism in different DNA forms, as well as potential new mechanisms for gene separations and sequencing. © 2012 IEEE.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Authors :	Name Email ORCID Mohamad, AS Jeynes, JC j.c.jeynes@surrey.ac.uk Hughes, MP m.hughes@surrey.ac.uk
Date :	22 November 2012
DOI :	10.1109/NANO.2012.6321928
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 12:38
Last Modified :	16 Jan 2019 18:33
URI:	http://epubs.surrey.ac.uk/id/eprint/836110

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