University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Label-free femtomolar detection of target DNA by impedimetric DNA sensor based on poly(pyrrole-nitrilotriacetic acid) film.

Baur, J, Gondran, C, Holzinger, M, Defrancq, E, Perrot, H and Cosnier, S (2010) Label-free femtomolar detection of target DNA by impedimetric DNA sensor based on poly(pyrrole-nitrilotriacetic acid) film. Anal Chem, 82 (3). pp. 1066-1072.

Full text not available from this repository.

Abstract

An ultrahigh performance impedimetric DNA sensor is presented showing detection limits in the femtomolar range. This electrochemical setup was constructed initially by electrogeneration of poly(11-pyrrol-1-yl-undecanoic acid N(alpha'),N(alpha)-bis(carboxymethyl)-L-lysine amide) (poly(pyrrole-NTA)) film. The latter was then modified by the coordination of Cu(2+) ions onto the chelating NTA centers followed by the immobilization of the ssHIV-DNA previously modified by a polyhistidine tag by affinity binding. The immobilization of the DNA probe and hybridization with the complementary target ssHIV-DNA were investigated using fluorescence microscopy and quantified with quartz crystal microbalance experiments leading to DNA probe and duplex coverage of 1.7 x 10(-11) and 7.7 x 10(-12) mol cm(-2), respectively. The duplex formation was corroborated by amperometric measurements through the duplex labeling by a glucose oxidase. In the presence of hydroquinone as redox indicator, the DNA sensor was applied to the impedimetric detection of target DNA without a labeling step. A linear quantification of the HIV DNA target was carried out in the range 10(-15) to 10(-8) mol L(-1).

Item Type: Article
Authors :
NameEmailORCID
Baur, Jj.baur@surrey.ac.ukUNSPECIFIED
Gondran, CUNSPECIFIEDUNSPECIFIED
Holzinger, MUNSPECIFIEDUNSPECIFIED
Defrancq, EUNSPECIFIEDUNSPECIFIED
Perrot, HUNSPECIFIEDUNSPECIFIED
Cosnier, SUNSPECIFIEDUNSPECIFIED
Date : 1 February 2010
Identification Number : https://doi.org/10.1021/ac9024329
Uncontrolled Keywords : Biosensing Techniques, Chelating Agents, Copper, DNA Probes, DNA, Viral, Electric Impedance, Electrochemical Techniques, Glucose Oxidase, HIV, Lysine, Microelectrodes, Nitrilotriacetic Acid, Nucleic Acid Hybridization, Pyrroles, Quartz
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 12:33
Last Modified : 17 May 2017 12:33
URI: http://epubs.surrey.ac.uk/id/eprint/835820

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800