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Semi-automated dielectrophoretic cell characterisation module for lab-on-chip applications

Kadri, NA, Fatoyinbo, HO, Hughes, MP and Labeed, FH (2011) Semi-automated dielectrophoretic cell characterisation module for lab-on-chip applications In: BIOMED 2011: 5th Kuala Lumpur International Conference on Biomedical Engineering, 2011-06-20 - 2011-06-23, Kuala Lumpur, Malaysia.

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Dielectrophoresis is an electrical phenomenon that occurs when a polarisable particle is placed in non-uniform electrical fields. The magnitude of the generated force is dependent upon the electrophysiological make-up of the particle, therefore the specific DEP profile may be attained for any polarisable particles based on the intrinsic electrical properties alone. Any changes to these parameters may be detected by observing the corresponding DEP spectra. Despite having the advantages of being non-invasive, DEP applications are still not widely used due to the time-consuming processes involved. This study presents the preliminary outcomes in the development of a semi-automated DEP-based cell characterisation tool that allowed concurrent DEP experiments to be conducted serially, thus significantly reducing the time taken to complete the required sets of experiments. The results showed that the system is capable of producing a DEP spectrum for K562 leukaemic cells between the 10 kHz to 1 MHz range in less than 10 minutes, when recorded at eight points per decade.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Divisions : Surrey research (other units)
Authors :
Kadri, NA
Date : 20 June 2011
DOI : 10.1007/978-3-642-21729-6_145
Contributors :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 12:07
Last Modified : 23 Jan 2020 17:37

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