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Inverse nickel-responsive regulation of two urease enzymes in the gastric pathogen Helicobacter mustelae

Stoof, J, Breijer, S, Pot, RG, van der Neut, D, Kuipers, EJ, Kusters, JG and van Vliet, AH (2008) Inverse nickel-responsive regulation of two urease enzymes in the gastric pathogen Helicobacter mustelae Environmental Microbiology, 10 (10). pp. 2586-2597.

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Abstract

The acidic gastric environment of mammals can be chronically colonized by pathogenic Helicobacter species, which use the nickel-dependent urea-degrading enzyme urease to confer acid resistance. Nickel availability in the mammal host is low, being mostly restricted to vegetarian dietary sources, and thus Helicobacter species colonizing carnivores may be subjected to episodes of nickel deficiency and associated acid sensitivity. The aim of this study was to investigate how these Helicobacter species have adapted to the nickel-restricted diet of their carnivorous host. Three carnivore-colonizing Helicobacter species express a second functional urea-degrading urease enzyme (UreA2B2), which functions as adaptation to nickel deficiency. UreA2B2 was not detected in seven other Helicobacter species, and is in Helicobacter mustelae only expressed in nickel-restricted conditions, and its expression was higher in iron-rich conditions. In contrast to the standard urease UreAB, UreA2B2 does not require activation by urease or hydrogenase accessory proteins, which mediate nickel incorporation into these enzymes. Activity of either UreAB or UreA2B2 urease allowed survival of a severe acid shock in the presence of urea, demonstrating a functional role for UreA2B2 in acid resistance. Pathogens often express colonization factors which are adapted to their host. The UreA2B2 urease could represent an example of pathogen adaptation to the specifics of the diet of their carnivorous host, rather than to the host itself.

Item Type: Article
Subjects : Veterinary Medicine
Authors :
NameEmailORCID
Stoof, JUNSPECIFIEDUNSPECIFIED
Breijer, SUNSPECIFIEDUNSPECIFIED
Pot, RGUNSPECIFIEDUNSPECIFIED
van der Neut, DUNSPECIFIEDUNSPECIFIED
Kuipers, EJUNSPECIFIEDUNSPECIFIED
Kusters, JGUNSPECIFIEDUNSPECIFIED
van Vliet, AHa.vanvliet@surrey.ac.ukUNSPECIFIED
Date : 28 June 2008
Identification Number : https://doi.org/10.1111/j.1462-2920.2008.01681.x
Copyright Disclaimer : © 2008 The Authors. Journal compilation © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:47
Last Modified : 18 May 2017 12:43
URI: http://epubs.surrey.ac.uk/id/eprint/829192

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