University of Surrey

Test tubes in the lab Research in the ATI Dance Research

PerR controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of Campylobacter jejuni

Handley, RA, Mulholland, F, Reuter, M, Ramachandran, VK, Musk, H, Clissold, L, Le Brun, NE and van Vliet, AH (2015) PerR controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of Campylobacter jejuni Microbiology, 161 (7). pp. 1524-1536.

Full text not available from this repository.

Abstract

The foodborne bacterial pathogen Campylobacter jejuni is an obligate microaerophile that is exposed to atmospheric oxygen during transmission through the food chain. Survival under aerobic conditions requires the concerted control of oxidative stress systems, which in C. jejuni are intimately connected with iron metabolism via the PerR and Fur regulatory proteins. Here, we have characterized the roles of C. jejuni PerR in oxidative stress and motility phenotypes, and its regulon at the level of transcription, protein expression and promoter interactions. Insertional inactivation of perR in the C. jejuni reference strains NCTC 11168, 81-176 and 81116 did not result in any growth deficiencies, but strongly increased survival in atmospheric oxygen conditions, and allowed growth around filter discs infused with up to 30 % H2O2 (8.8 M). Expression of catalase, alkyl hydroperoxide reductase, thioredoxin reductase and the Rrc desulforubrerythrin was increased in the perR mutant, and this was mediated at the transcriptional level as shown by electrophoretic mobility shift assays of the katA, ahpC and trxB promoters using purified PerR. Differential RNA-sequencing analysis of a fur perR mutant allowed the identification of eight previously unknown transcription start sites of genes controlled by Fur and/or PerR. Finally, inactivation of perR in C. jejuni did not result in reduced motility, and did not reduce killing of Galleria melonella wax moth larvae. In conclusion, PerR plays an important role in controlling oxidative stress resistance and aerobic survival of C. jejuni, but this role does not extend into control of motility and associated phenotypes.

Item Type: Article
Subjects : Veterinary Medicine
Authors :
NameEmailORCID
Handley, RAUNSPECIFIEDUNSPECIFIED
Mulholland, FUNSPECIFIEDUNSPECIFIED
Reuter, MUNSPECIFIEDUNSPECIFIED
Ramachandran, VKUNSPECIFIEDUNSPECIFIED
Musk, HUNSPECIFIEDUNSPECIFIED
Clissold, LUNSPECIFIEDUNSPECIFIED
Le Brun, NEUNSPECIFIEDUNSPECIFIED
van Vliet, AHa.vanvliet@surrey.ac.ukUNSPECIFIED
Date : 1 July 2015
Identification Number : https://doi.org/10.1099/mic.0.000109
Copyright Disclaimer : © 2015 The Authors. Published by the Microbiology Society
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:46
Last Modified : 18 May 2017 12:43
URI: http://epubs.surrey.ac.uk/id/eprint/829183

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800