University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Applications of High-Throughput Clonogenic Survival Assays in High-LET Particle Microbeams.

Georgantzoglou, A, Merchant, MJ, Jeynes, JC, Mayhead, N, Punia, N, Butler, RE and Jena, R (2015) Applications of High-Throughput Clonogenic Survival Assays in High-LET Particle Microbeams. Front Oncol, 5.

Full text not available from this repository.

Abstract

Charged particle therapy is increasingly becoming a valuable tool in cancer treatment, mainly due to the favorable interaction of particle radiation with matter. Its application is still limited due, in part, to lack of data regarding the radiosensitivity of certain cell lines to this radiation type, especially to high-linear energy transfer (LET) particles. From the earliest days of radiation biology, the clonogenic survival assay has been used to provide radiation response data. This method produces reliable data but it is not optimized for high-throughput microbeam studies with high-LET radiation where high levels of cell killing lead to a very low probability of maintaining cells' clonogenic potential. A new method, therefore, is proposed in this paper, which could potentially allow these experiments to be conducted in a high-throughput fashion. Cells are seeded in special polypropylene dishes and bright-field illumination provides cell visualization. Digital images are obtained and cell detection is applied based on corner detection, generating individual cell targets as x-y points. These points in the dish are then irradiated individually by a micron field size high-LET microbeam. Post-irradiation, time-lapse imaging follows cells' response. All irradiated cells are tracked by linking trajectories in all time-frames, based on finding their nearest position. Cell divisions are detected based on cell appearance and individual cell temporary corner density. The number of divisions anticipated is low due to the high probability of cell killing from high-LET irradiation. Survival curves are produced based on cell's capacity to divide at least four to five times. The process is repeated for a range of doses of radiation. Validation shows the efficiency of the proposed cell detection and tracking method in finding cell divisions.

Item Type: Article
Authors :
NameEmailORCID
Georgantzoglou, AUNSPECIFIEDUNSPECIFIED
Merchant, MJUNSPECIFIEDUNSPECIFIED
Jeynes, JCUNSPECIFIEDUNSPECIFIED
Mayhead, NUNSPECIFIEDUNSPECIFIED
Punia, NUNSPECIFIEDUNSPECIFIED
Butler, REr.shrimpton@surrey.ac.ukUNSPECIFIED
Jena, RUNSPECIFIEDUNSPECIFIED
Date : 2015
Identification Number : https://doi.org/10.3389/fonc.2015.00305
Uncontrolled Keywords : bright-field imaging, cell tracking, clonogenic survival assay, high-LET radiation, microbeam
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:44
Last Modified : 17 May 2017 14:52
URI: http://epubs.surrey.ac.uk/id/eprint/829006

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800