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Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model

Ciesielczuk, H, Betts, J, Phee, L, Hope, R, Doumith, M, Woodford, N and Wareham, DW (2015) Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model Virulence, 6 (2). pp. 145-151.

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Abstract

Extra-intestinal pathogenic Escherichia coli (ExPEC) are a significant cause of urinary tract infections and bacteraemia worldwide. Currently no single virulence factor or ExPEC lineage has been identified as the sole contributor to severe extra-intestinal infection and/or urosepsis. Galleria mellonella has recently been established as a simple model for studying the comparative virulence of ExPEC. In this study we investigated the virulence of 40 well-characterized ExPEC strains, in G. mellonella, by measuring mortality (larvae survival), immune recognition/response (melanin production) and cell damage (lactate dehydrogenase production). Although mortality was similar between urinary and bloodstream isolates, it was heightened for community-associated infections, complicated UTIs and urinary-source bacteraemia. Isolates of ST131 and those possessing afa/dra, ompT and serogroup O6 were also associated with heightened virulence.

Item Type: Article
Authors :
NameEmailORCID
Ciesielczuk, HUNSPECIFIEDUNSPECIFIED
Betts, Jjono.betts@surrey.ac.ukUNSPECIFIED
Phee, LUNSPECIFIEDUNSPECIFIED
Hope, RUNSPECIFIEDUNSPECIFIED
Doumith, MUNSPECIFIEDUNSPECIFIED
Woodford, NUNSPECIFIEDUNSPECIFIED
Wareham, DWUNSPECIFIEDUNSPECIFIED
Date : 5 February 2015
Identification Number : 10.4161/21505594.2014.988095
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:43
Last Modified : 17 May 2017 14:52
URI: http://epubs.surrey.ac.uk/id/eprint/828980

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