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The human PEX3 gene encoding a peroxisomal assembly protein: genomic organization, positional mapping, and mutation analysis in candidate phenotypes

Muntau, AC, Holzinger, A, Mayerhofer, PU, Gartner, J, Roscher, AA and Kammerer, S (2000) The human PEX3 gene encoding a peroxisomal assembly protein: genomic organization, positional mapping, and mutation analysis in candidate phenotypes Biochem Biophys Res Commun, 268. pp. 704-710.

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Abstract

In yeasts, the peroxin Pex3p was identified as a peroxisomal integral membrane protein that presumably plays a role in the early steps of peroxisomal assembly. In humans, defects of peroxins cause peroxisomal biogenesis disorders such as Zellweger syndrome. We previously reported data on the human PEX3 cDNA and its protein, which in addition to the peroxisomal targeting sequence contains a putative endoplasmic reticulum targeting signal. Here we report the genomic organization, sequencing of the putative promoter region, chromosomal localization, and physical mapping of the human PEX3 gene. The gene is composed of 12 exons and 11 introns spanning a region of approximately 40 kb. The highly conserved putative promoter region is very GC rich, lacks typical TATA and CCAAT boxes, and contains potential Sp1, AP1, and AP2 binding sites. The gene was localized to chromosome 6q23-24 and D6S279 was identified to be the closest positional marker. As yeast mutants deficient in PEX3 have been shown to lack peroxisomes as well as any peroxisomal remnant structures, human PEX3 is a candidate gene for peroxisomal assembly disorders. Mutation analysis of the human PEX3 gene was therefore performed in fibroblasts from patients suffering from peroxisome biogenesis disorders. Complementation groups 1, 4, 7, 8, and 9 according to the numbering system of Kennedy Krieger Institute were analyzed but no difference to the wild-type sequence was detected. PEX3 mutations were therefore excluded as the molecular basis of the peroxisomal defect in these complementation groups.

Item Type: Article
Authors :
NameEmailORCID
Muntau, ACUNSPECIFIEDUNSPECIFIED
Holzinger, AUNSPECIFIEDUNSPECIFIED
Mayerhofer, PUp.mayerhofer@surrey.ac.ukUNSPECIFIED
Gartner, JUNSPECIFIEDUNSPECIFIED
Roscher, AAUNSPECIFIEDUNSPECIFIED
Kammerer, SUNSPECIFIEDUNSPECIFIED
Date : 2000
Identification Number : https://doi.org/10.1006/bbrc.2000.2193
Uncontrolled Keywords : *ATP-Binding Cassette Transporters Base Sequence Chromosome Mapping Chromosomes, Artificial, Yeast/genetics Chromosomes, Human, Pair 6/genetics Cloning, Molecular DNA Mutational Analysis DNA Primers/genetics Exons Fungal Proteins/genetics Genetic Complementation Test Humans In Situ Hybridization, Fluorescence Introns Lipoproteins/*genetics Membrane Proteins/*genetics Molecular Sequence Data Peroxisomal Disorders/genetics Peroxisomes/*metabolism Phenotype Promoter Regions, Genetic Saccharomyces cerevisiae/genetics *Saccharomyces cerevisiae Proteins
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:42
Last Modified : 17 May 2017 14:51
URI: http://epubs.surrey.ac.uk/id/eprint/828862

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