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Genomic organization, expression analysis, and chromosomal localization of the mouse PEX3 gene encoding a peroxisomal assembly protein

Muntau, AC, Mayerhofer, PU, Albet, S, Schmid, TE, Bugaut, M, Roscher, AA and Kammerer, S (2000) Genomic organization, expression analysis, and chromosomal localization of the mouse PEX3 gene encoding a peroxisomal assembly protein Biol Chem, 381. pp. 337-342.

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Abstract

The peroxin Pex3p has been identified as an integral peroxisomal membrane protein in yeast where pex3 mutants lack peroxisomal remnant structures. Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested. We report here the cDNA cloning and the genomic structure of the mouse PEX3 gene. The 2 kb cDNA encodes a polypeptide of 372 amino acids (42 kDa). The gene spans a region of 30 kb, contains 12 exons and 11 introns and is located on band A of chromosome 10. The putative promoter region exhibits characteristic housekeeping features. PEX3 expression was identified in all tissues analyzed, with the strongest signals in liver and in testis, and could not be induced by fenofibrate. The data presented may be useful for the generation of a mouse model defective in PEX3 in order to clarify the yet unknown functional impact of disturbances in early peroxisomal membrane assembly.

Item Type: Article
Authors :
NameEmailORCID
Muntau, ACUNSPECIFIEDUNSPECIFIED
Mayerhofer, PUp.mayerhofer@surrey.ac.ukUNSPECIFIED
Albet, SUNSPECIFIEDUNSPECIFIED
Schmid, TEUNSPECIFIEDUNSPECIFIED
Bugaut, MUNSPECIFIEDUNSPECIFIED
Roscher, AAUNSPECIFIEDUNSPECIFIED
Kammerer, SUNSPECIFIEDUNSPECIFIED
Date : 2000
Identification Number : https://doi.org/10.1515/BC.2000.044
Uncontrolled Keywords : *ATP-Binding Cassette Transporters Animals *Chromosome Mapping Cloning, Molecular DNA, Complementary Exons/genetics Fenofibrate/pharmacology Fungal Proteins/*genetics Gene Expression/*genetics *Genome Introns/genetics Membrane Proteins/*genetics Mice Molecular Sequence Data Peptide Biosynthesis/genetics Peroxisomes/*chemistry/metabolism Polymerase Chain Reaction Promoter Regions, Genetic/genetics RNA, Messenger/drug effects/metabolism *Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Tissue Distribution
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:42
Last Modified : 17 May 2017 14:51
URI: http://epubs.surrey.ac.uk/id/eprint/828860

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