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First identification of methicillin-resistant Staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in Trinidad, West Indies.

Dziva, F, Wint, C, Auguste, T, Heeraman, C, Dacon, C, Yu, P and Koma, LM (2015) First identification of methicillin-resistant Staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in Trinidad, West Indies. Infect Ecol Epidemiol, 5.

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Abstract

BACKGROUND: Otitis externa is a common inflammatory ear disease in dogs caused by a variety of pathogens, and coagulase-positive staphylococci are frequently isolated from such infections. OBJECTIVE: To identify antimicrobial susceptibility profiles and methicillin-resistant strains among coagulase-positive staphylococci isolated from otitis externa in dogs. METHODS: A cross-sectional study was performed over 2 years on 114 client-owned dogs presented to the Veterinary Teaching Hospital with a primary complaint of ear infections. Swabs were obtained from both ears and cultured for staphylococci which were subsequently confirmed as coagulase-positive using rabbit plasma. Antimicrobial susceptibility assays were assessed on all isolates followed by subsequent genetic analysis for species identification and detection of the mecA gene. RESULTS: Sixty-five coagulase-positive staphylococci were isolated from 114 client-owned dogs. The isolates exhibited resistance against neomycin (58.5%), streptomycin (49.2%), penicillin (49.2%), polymyxin B (44.6%), tetracycline (36.9%), sulphamethoxazole/trimethoprim (33.8%), kanamycin (33.8%), doxycycline (32.3%), norfloxacin (23.1%), amoxicillin/clavulanate (20%), ciprofloxacin (20%), enrofloxacin (18.5%), gentamicin (16.9%), and cephalothin (9.2%). Forty (61.5%) of the isolates were resistant to at least three or more antimicrobials and 10 were sensitive to all. Using a multiplex polymerase chain reaction assay based on species-specific regions of the thermonuclease (nuc) gene, 38/65 (58.5%) isolates were classified as Staphylococcus aureus, 23/65 (35.4%) as S. pseudintermedius, 2/65 (3.1%) as S. intermedius, and 2/65 (3.1%) as S. schleiferi. Analysis for the mecA gene revealed two positive isolates of S. pseudintermedius which were oxacillin-resistant, representing a first report of such organisms in the Caribbean. CONCLUSION: Despite the relatively high prevalence of multidrug-resistant coagulase-positive staphylococci in Trinidad, these are largely susceptible to gentamicin consistent with use in clinical practice. The first detection of methicillin-resistant S. pseudintermedius (MRSP) in dogs is likely to have implications on the treatment options for otitis externa in dogs and potential public health significance.

Item Type: Article
Authors :
NameEmailORCID
Dziva, Ff.dziva@surrey.ac.ukUNSPECIFIED
Wint, CUNSPECIFIEDUNSPECIFIED
Auguste, TUNSPECIFIEDUNSPECIFIED
Heeraman, CUNSPECIFIEDUNSPECIFIED
Dacon, CUNSPECIFIEDUNSPECIFIED
Yu, PUNSPECIFIEDUNSPECIFIED
Koma, LMUNSPECIFIEDUNSPECIFIED
Date : 2015
Uncontrolled Keywords : PCR, antimicrobials, mecA, otitis externa, staphylococci
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:40
Last Modified : 17 May 2017 10:40
URI: http://epubs.surrey.ac.uk/id/eprint/828763

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