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A fast and accurate method for controlling the correct labeling of products containing buffalo meat using High Resolution Melting (HRM) analysis.

Sakaridis, I, Ganopoulos, I, Argiriou, A and Tsaftaris, A (2013) A fast and accurate method for controlling the correct labeling of products containing buffalo meat using High Resolution Melting (HRM) analysis. Meat Sci, 94 (1). pp. 84-88.

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Abstract

The substitution of high priced meat with low cost ones and the fraudulent labeling of meat products make the identification and traceability of meat species and their processed products in the food chain important. A polymerase chain reaction followed by a High Resolution Melting (HRM) analysis was developed for species specific detection of buffalo; it was applied in six commercial meat products. A pair of specific 12S and universal 18S rRNA primers were employed and yielded DNA fragments of 220bp and 77bp, respectively. All tested products were found to contain buffalo meat and presented melting curves with at least two visible inflection points derived from the amplicons of the 12S specific and 18S universal primers. The presence of buffalo meat in meat products and the adulteration of buffalo products with unknown species were established down to a level of 0.1%. HRM was proven to be a fast and accurate technique for authentication testing of meat products.

Item Type: Article
Authors :
NameEmailORCID
Sakaridis, Ii.sakaridis@surrey.ac.ukUNSPECIFIED
Ganopoulos, IUNSPECIFIEDUNSPECIFIED
Argiriou, AUNSPECIFIEDUNSPECIFIED
Tsaftaris, AUNSPECIFIEDUNSPECIFIED
Date : May 2013
Identification Number : https://doi.org/10.1016/j.meatsci.2012.12.017
Uncontrolled Keywords : Animals, Buffaloes, DNA, DNA Primers, Deception, Diet, Food Analysis, Food Labeling, Freezing, Genotyping Techniques, Humans, Meat Products, Polymerase Chain Reaction, RNA, Ribosomal, RNA, Ribosomal, 18S, Reproducibility of Results, Species Specificity
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:39
Last Modified : 17 May 2017 14:51
URI: http://epubs.surrey.ac.uk/id/eprint/828681

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