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EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells.

Dziva, F, Mahajan, A, Cameron, P, Currie, C, McKendrick, IJ, Wallis, TS, Smith, DG and Stevens, MP (2007) EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells. FEMS Microbiol Lett, 271 (2). pp. 258-264.

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Abstract

Enterohaemorrhagic Escherichia coli (EHEC) comprise a group of zoonotic diarrhoeal pathogens of worldwide importance. Cattle are a key reservoir; however the molecular mechanisms that promote persistent colonization of the bovine intestines by EHEC are ill-defined. The large plasmid of EHEC O157:H7 encodes several putative virulence factors. Here, it is reported that the pO157-encoded Type V-secreted serine protease EspP influences the intestinal colonization of calves. To dissect the basis of attenuation, a bovine primary rectal epithelial cell line was developed. Adherence of E. coli O157:H7 to such cells was significantly impaired by espP mutation but restored upon addition of highly purified exogenous EspP. Data of this study add to the growing body of evidence that cytotoxins facilitate intestinal colonization by EHEC.

Item Type: Article
Authors :
NameEmailORCID
Dziva, Ff.dziva@surrey.ac.ukUNSPECIFIED
Mahajan, AUNSPECIFIEDUNSPECIFIED
Cameron, PUNSPECIFIEDUNSPECIFIED
Currie, CUNSPECIFIEDUNSPECIFIED
McKendrick, IJUNSPECIFIEDUNSPECIFIED
Wallis, TSUNSPECIFIEDUNSPECIFIED
Smith, DGUNSPECIFIEDUNSPECIFIED
Stevens, MPUNSPECIFIEDUNSPECIFIED
Date : June 2007
Identification Number : 10.1111/j.1574-6968.2007.00724.x
Uncontrolled Keywords : Animals, Bacterial Adhesion, Cattle, Cells, Cultured, Epithelial Cells, Escherichia coli O157, Escherichia coli Proteins, Intestinal Mucosa, Mutation, Serine Endopeptidases
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:39
Last Modified : 17 May 2017 10:39
URI: http://epubs.surrey.ac.uk/id/eprint/828672

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