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Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.

Eckert, SE, Dziva, F, Chaudhuri, RR, Langridge, GC, Turner, DJ, Pickard, DJ, Maskell, DJ, Thomson, NR and Stevens, MP (2011) Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle. J Bacteriol, 193 (7). pp. 1771-1776.

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Abstract

Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.

Item Type: Article
Authors :
NameEmailORCID
Eckert, SEUNSPECIFIEDUNSPECIFIED
Dziva, Ff.dziva@surrey.ac.ukUNSPECIFIED
Chaudhuri, RRUNSPECIFIEDUNSPECIFIED
Langridge, GCUNSPECIFIEDUNSPECIFIED
Turner, DJUNSPECIFIEDUNSPECIFIED
Pickard, DJUNSPECIFIEDUNSPECIFIED
Maskell, DJUNSPECIFIEDUNSPECIFIED
Thomson, NRUNSPECIFIEDUNSPECIFIED
Stevens, MPUNSPECIFIEDUNSPECIFIED
Date : April 2011
Identification Number : https://doi.org/10.1128/JB.01292-10
Uncontrolled Keywords : Animals, Cattle, Cattle Diseases, Chromosomes, Bacterial, DNA Transposable Elements, DNA, Bacterial, Escherichia coli O157, Genetic Fitness, Genotype, Mutagenesis, Insertional, Plasmids
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:39
Last Modified : 17 May 2017 10:39
URI: http://epubs.surrey.ac.uk/id/eprint/828663

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