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A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli in chickens.

Sadeyen, JR, Kaiser, P, Stevens, MP and Dziva, F (2015) A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli in chickens. Vaccine, 33 (31). pp. 3624-3627.

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Abstract

Avian pathogenic Escherichia coli (APEC) exert substantial economic costs on poultry producers worldwide. Vaccination is an attractive method of control, but the immunological basis of protection is poorly understood. Here, we examine the effect of intramuscular injection of cyclophosphamide or saline on homologous protection induced by licensed inactivated or live-attenuated APEC O78 vaccines in chickens. In saline-treated birds, both vaccines induced significant APEC-specific IgY and protection against homologous challenge, as evidenced by enumeration of tissue-associated bacteria and analysis of pathology. In cyclophosphamide-treated birds, B cells were severely depleted whereas percentages of circulating CD4- and CD8-positive T cells were normal as detected by flow cytometry. Further, such birds did not produce APEC-specific IgY and were as susceptible to challenge as age-matched unvaccinated controls. The data indicate that homologous protection conferred by licensed APEC vaccines strictly requires a cyclophosphamide-sensitive cell population that includes B cells.

Item Type: Article
Authors :
NameEmailORCID
Sadeyen, JRUNSPECIFIEDUNSPECIFIED
Kaiser, PUNSPECIFIEDUNSPECIFIED
Stevens, MPUNSPECIFIEDUNSPECIFIED
Dziva, Ff.dziva@surrey.ac.ukUNSPECIFIED
Date : 17 July 2015
Identification Number : 10.1016/j.vaccine.2015.06.034
Uncontrolled Keywords : APEC, Antibody, Bursectomy, Chicken, Cyclophosphamide, Vaccination, Animals, Antibodies, Bacterial, B-Lymphocytes, Bacterial Load, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Chickens, Cyclophosphamide, Escherichia coli, Escherichia coli Infections, Escherichia coli Vaccines, Immunoglobulins, Injections, Intramuscular, Poultry Diseases, Vaccines, Attenuated, Vaccines, Inactivated
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:39
Last Modified : 17 May 2017 10:39
URI: http://epubs.surrey.ac.uk/id/eprint/828656

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