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The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin.

Marie, H, Pratt, SJ, Betson, M, Epple, H, Kittler, JT, Meek, L, Moss, SJ, Troyanovsky, S, Attwell, D, Longmore, GD and Braga, VM (2003) The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin. J Biol Chem, 278 (2). pp. 1220-1228.

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Cell-cell adhesive events affect cell growth and fate decisions and provide spatial clues for cell polarity within tissues. The complete molecular determinants required for adhesive junction formation and their function are not completely understood. LIM domain-containing proteins have been shown to be present at cell-cell contact sites and are known to shuttle into the nucleus where they can affect cell fate and growth; however, their precise localization at cell-cell contacts, how they localize to these sites, and what their functions are at these sites is unknown. Here we show that, in primary keratinocytes, the LIM domain protein Ajuba is recruited to cadherin-dependent cell-cell adhesive complexes in a regulated manner. At cadherin adhesive complexes Ajuba interacts with alpha-catenin, and alpha-catenin is required for efficient recruitment of Ajuba to cell junctions. Ajuba also interacts directly with F-actin. Keratinocytes from Ajuba null mice exhibit abnormal cell-cell junction formation and/or stability and function. These data reveal Ajuba as a new component at cadherin-mediated cell-cell junctions and suggest that Ajuba may contribute to the bridging of the cadherin adhesive complexes to the actin cytoskeleton and as such contribute to the formation or strengthening of cadherin-mediated cell-cell adhesion.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Marie, H
Pratt, SJ
Epple, H
Kittler, JT
Meek, L
Moss, SJ
Troyanovsky, S
Attwell, D
Longmore, GD
Braga, VM
Date : 10 January 2003
DOI : 10.1074/jbc.M205391200
Uncontrolled Keywords : Actins, Cadherins, Cell Adhesion, Cell Communication, Cytoskeletal Proteins, Homeodomain Proteins, Humans, Intercellular Junctions, Keratinocytes, LIM Domain Proteins, alpha Catenin
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:33
Last Modified : 24 Jan 2020 19:34

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