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MKAN27435 is required for the biosynthesis of higher subclasses of lipooligosaccharides in Mycobacterium kansasii.

Nataraj, V, Pang, PC, Haslam, SM, Veerapen, N, Minnikin, DE, Dell, A, Besra, GS and Bhatt, A (2015) MKAN27435 is required for the biosynthesis of higher subclasses of lipooligosaccharides in Mycobacterium kansasii. PLoS One, 10 (3).

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Abstract

Lipooligosaccharides are glycolipids found in the cell wall of many mycobacterial species including the opportunistic pathogen Mycobacterium kansasii. The genome of M. kansasii ATCC12478 contains a cluster with genes orthologous to Mycobacterium marinum LOS biosynthesis genes. To initiate a genetic dissection of this cluster and demonstrate its role in LOS biosynthesis in M. kansasii, we chose MKAN27435, a gene encoding a putative glycosyltransferase. Using Specialized Transduction, a phage-based gene knockout tool previously used to generate null mutants in other mycobacteria, we generated a MKAN27435 null mutant. The mutant strain was found to be defective in the biosynthesis of higher LOS subspecies, viz LOS-IV, LOS-V, LOS-VI and LOS-VII. Additionally, a range of low abundance species were detected in the mutant strain and mass spectroscopic analysis indicated that these were shunt products generated from LOS-III by the addition of up to six molecules of a pentose.

Item Type: Article
Authors :
NameEmailORCID
Nataraj, Vv.nataraj@surrey.ac.ukUNSPECIFIED
Pang, PCUNSPECIFIEDUNSPECIFIED
Haslam, SMUNSPECIFIEDUNSPECIFIED
Veerapen, NUNSPECIFIEDUNSPECIFIED
Minnikin, DEUNSPECIFIEDUNSPECIFIED
Dell, AUNSPECIFIEDUNSPECIFIED
Besra, GSUNSPECIFIEDUNSPECIFIED
Bhatt, AUNSPECIFIEDUNSPECIFIED
Date : 2015
Identification Number : https://doi.org/10.1371/journal.pone.0122804
Uncontrolled Keywords : Gene Knockout Techniques, Glycosyltransferases, Lipopolysaccharides, Multigene Family, Mutation, Mycobacterium kansasii, Open Reading Frames
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:31
Last Modified : 17 May 2017 10:31
URI: http://epubs.surrey.ac.uk/id/eprint/828124

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