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APE-type non-LTR retrotransposons of multicellular organisms encode virus-like 2A oligopeptide sequences, which mediate translational recoding during protein synthesis.

Odon, V, Luke, GA, Roulston, C, de Felipe, P, Ruan, L, Escuin-Ordinas, H, Brown, JD, Ryan, MD and Sukhodub, A (2013) APE-type non-LTR retrotransposons of multicellular organisms encode virus-like 2A oligopeptide sequences, which mediate translational recoding during protein synthesis. Mol Biol Evol, 30 (8). pp. 1955-1965.

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Abstract

2A oligopeptide sequences ("2As") mediate a cotranslational recoding event termed "ribosome skipping." Previously we demonstrated the activity of 2As (and "2A-like sequences") within a wide range of animal RNA virus genomes and non-long terminal repeat retrotransposons (non-LTRs) in the genomes of the unicellular organisms Trypanosoma brucei (Ingi) and T. cruzi (L1Tc). Here, we report the presence of 2A-like sequences in the genomes of a wide range of multicellular organisms and, as in the trypanosome genomes, within non-LTR retrotransposons (non-LTRs)-clustering in the Rex1, Crack, L2, L2A, and CR1 clades, in addition to Ingi. These 2A-like sequences were tested for translational recoding activity, and highly active sequences were found within the Rex1, L2, CR1, and Ingi clades. The presence of 2A-like sequences within non-LTRs may not only represent a method of controlling protein biogenesis but also shows some correlation with such apurinic/apyrimidinic DNA endonuclease-type non-LTRs encoding one, rather than two, open reading frames (ORFs). Interestingly, such non-LTRs cluster with closely related elements lacking 2A-like recoding elements but retaining ORF1. Taken together, these observations suggest that acquisition of 2A-like translational recoding sequences may have played a role in the evolution of these elements.

Item Type: Article
Authors :
NameEmailORCID
Odon, Vv.odon@surrey.ac.ukUNSPECIFIED
Luke, GAUNSPECIFIEDUNSPECIFIED
Roulston, CUNSPECIFIEDUNSPECIFIED
de Felipe, PUNSPECIFIEDUNSPECIFIED
Ruan, LUNSPECIFIEDUNSPECIFIED
Escuin-Ordinas, HUNSPECIFIEDUNSPECIFIED
Brown, JDUNSPECIFIEDUNSPECIFIED
Ryan, MDUNSPECIFIEDUNSPECIFIED
Sukhodub, AUNSPECIFIEDUNSPECIFIED
Date : August 2013
Identification Number : 10.1093/molbev/mst102
Uncontrolled Keywords : 2A-like sequences, APE-type non-LTR, retrotransposon, translational recoding, Amino Acid Sequence, Genome, Protozoan, Molecular Sequence Data, Oligopeptides, Open Reading Frames, Phylogeny, Protein Biosynthesis, Retroelements, Sequence Alignment, Trypanosoma
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:29
Last Modified : 17 May 2017 14:49
URI: http://epubs.surrey.ac.uk/id/eprint/827962

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