University of Surrey

Test tubes in the lab Research in the ATI Dance Research

mTOR signaling regulates the processing of pre-rRNA in human cells.

Iadevaia, V, Zhang, Z, Jan, E and Proud, CG (2012) mTOR signaling regulates the processing of pre-rRNA in human cells. Nucleic Acids Res, 40 (6). pp. 2527-2539.

Full text not available from this repository.

Abstract

Signaling through the mammalian target of rapamycin, complex 1 (mTORC1), positively regulates the transcription of ribosomal RNA (rRNA) and the synthesis of ribosomal proteins, thereby promoting the complex process of ribosome biogenesis. The major rRNAs are transcribed as a single precursor, which must be processed to create the 5.8S, 18S and 28S rRNAs. We used a new non-radioactive labeling approach to study the effects of rapamycin, an inhibitor of mTORC1, on rRNA synthesis. Rapamycin not only impaired synthesis of new 18S, 28S or 5S rRNA but also induced their decay. This prompted us to examine the effects of rapamycin on rRNA processing. We show that rapamycin also interferes with the processing events that generate 18S and 28S rRNA. rRNA transcription and processing occur in regions of the nucleus known as nucleoli. We find that the mTORC1 components raptor and mTOR are both present in nucleoli, where they may regulate rRNA maturation events. While rapamycin has no effect on overall nucleolar morphology or its proteome, it does induce loss of mTOR and raptor from them. These data show that mTORC1 is located in nucleoli where it acts to regulate events involved in ribosome biogenesis including the maturation of rRNA molecules.

Item Type: Article
Authors :
NameEmailORCID
Iadevaia, Vv.iadevaia@surrey.ac.ukUNSPECIFIED
Zhang, ZUNSPECIFIEDUNSPECIFIED
Jan, EUNSPECIFIEDUNSPECIFIED
Proud, CGUNSPECIFIEDUNSPECIFIED
Date : March 2012
Identification Number : https://doi.org/10.1093/nar/gkr1040
Uncontrolled Keywords : Cell Nucleolus, HeLa Cells, Humans, Multiprotein Complexes, Protein Biosynthesis, Proteins, RNA Precursors, RNA Processing, Post-Transcriptional, RNA Stability, RNA, Ribosomal, Ribosomal Proteins, Signal Transduction, Sirolimus, TOR Serine-Threonine Kinases
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:28
Last Modified : 17 May 2017 14:49
URI: http://epubs.surrey.ac.uk/id/eprint/827938

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800