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Roles of the mammalian target of rapamycin, mTOR, in controlling ribosome biogenesis and protein synthesis.

Iadevaia, V, Huo, Y, Zhang, Z, Foster, LJ and Proud, CG (2012) Roles of the mammalian target of rapamycin, mTOR, in controlling ribosome biogenesis and protein synthesis. Biochem Soc Trans, 40 (1). pp. 168-172.

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Abstract

mTORC1 (mammalian target of rapamycin complex 1) is controlled by diverse signals (e.g. hormones, growth factors, nutrients and cellular energy status) and regulates a range of processes including anabolic metabolism, cell growth and cell division. We have studied the impact of inhibiting mTOR on protein synthesis in human cells. Partial inhibition of mTORC1 by rapamycin has only a limited impact on protein synthesis, but inhibiting mTOR kinase activity causes much greater inhibition of protein synthesis. Using a pulsed stable-isotope-labelling technique, we show that the rapamycin and mTOR (mammalian target of rapamycin) kinase inhibitors have differential effects on the synthesis of specific proteins. In particular, the synthesis of proteins encoded by mRNAs that have a 5'-terminal pyrimidine tract is strongly inhibited by mTOR kinase inhibitors. Many of these mRNAs encode ribosomal proteins. mTORC1 also promotes the synthesis of rRNA, although the mechanisms involved remain to be clarified. We found that mTORC1 also regulates the processing of the precursors of rRNA. mTORC1 thus co-ordinates several steps in ribosome biogenesis.

Item Type: Article
Authors :
NameEmailORCID
Iadevaia, Vv.iadevaia@surrey.ac.ukUNSPECIFIED
Huo, YUNSPECIFIEDUNSPECIFIED
Zhang, ZUNSPECIFIEDUNSPECIFIED
Foster, LJUNSPECIFIEDUNSPECIFIED
Proud, CGUNSPECIFIEDUNSPECIFIED
Date : February 2012
Identification Number : 10.1042/BST20110682
Uncontrolled Keywords : Animals, Gene Expression Regulation, Enzymologic, Humans, Multiprotein Complexes, Protein Biosynthesis, Proteins, Ribosomes, Sirolimus, TOR Serine-Threonine Kinases, Transcription Factors
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:28
Last Modified : 17 May 2017 14:49
URI: http://epubs.surrey.ac.uk/id/eprint/827936

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