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Response to arsenate treatment in Schizosaccharomyces pombe and the role of its arsenate reductase activity.

Salgado, A, López-Serrano Oliver, A, Matia-González, AM, Sotelo, J, Zarco-Fernández, S, Muñoz-Olivas, R, Cámara, C and Rodríguez-Gabriel, MA (2012) Response to arsenate treatment in Schizosaccharomyces pombe and the role of its arsenate reductase activity. PLoS One, 7 (8).

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Abstract

Arsenic toxicity has been studied for a long time due to its effects in humans. Although epidemiological studies have demonstrated multiple effects in human physiology, there are many open questions about the cellular targets and the mechanisms of response to arsenic. Using the fission yeast Schizosaccharomyces pombe as model system, we have been able to demonstrate a strong activation of the MAPK Spc1/Sty1 in response to arsenate. This activation is dependent on Wis1 activation and Pyp2 phosphatase inactivation. Using arsenic speciation analysis we have also demonstrated the previously unknown capacity of S. pombe cells to reduce As (V) to As (III). Genetic analysis of several fission yeast mutants point towards the cell cycle phosphatase Cdc25 as a possible candidate to carry out this arsenate reductase activity. We propose that arsenate reduction and intracellular accumulation of arsenite are the key mechanisms of arsenate tolerance in fission yeast.

Item Type: Article
Authors :
NameEmailORCID
Salgado, AUNSPECIFIEDUNSPECIFIED
López-Serrano Oliver, AUNSPECIFIEDUNSPECIFIED
Matia-González, AMUNSPECIFIEDUNSPECIFIED
Sotelo, JUNSPECIFIEDUNSPECIFIED
Zarco-Fernández, SUNSPECIFIEDUNSPECIFIED
Muñoz-Olivas, RUNSPECIFIEDUNSPECIFIED
Cámara, CUNSPECIFIEDUNSPECIFIED
Rodríguez-Gabriel, MAUNSPECIFIEDUNSPECIFIED
Date : 2012
Identification Number : 10.1371/journal.pone.0043208
Uncontrolled Keywords : Arsenate Reductases, Arsenates, Cell Cycle Proteins, Chromatography, Ion Exchange, Chromatography, Liquid, Genotype, Immunoblotting, Mass Spectrometry, Mitogen-Activated Protein Kinase Kinases, Mitogen-Activated Protein Kinases, Phosphoprotein Phosphatases, Protein Tyrosine Phosphatases, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Spectrophotometry, Atomic
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:26
Last Modified : 17 May 2017 10:26
URI: http://epubs.surrey.ac.uk/id/eprint/827812

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