University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Patterns of Onchocerca volvulus recombinant antigen recognition in a bovine model of onchocerciasis.

Graham, SP, Wu, Y, Henkle-Duehrsen, K, Lustigman, S, Unnasch, TR, Braun, G, Williams, SA, McCarthy, J, Trees, AJ and Bianco, AE (1999) Patterns of Onchocerca volvulus recombinant antigen recognition in a bovine model of onchocerciasis. Parasitology, 119 ( . pp. 603-612.

Full text not available from this repository.

Abstract

The antibody responses of 8 cattle experimentally infected with Onchocerca ochengi to 18 recombinant O. volvulus antigens were measured by ELISA. In addition to establishing antigenic cross-reactivity between the species, the dynamics of antigen-specific responses were examined to assess how the recognition of the antigens compared to the known stage-specificity of expression. Six cattle responded to all of the antigens and 2 animals responded to all but 1. The dynamics of the recognition of 4 antigens (B20, MOv-2, MOv-14 and OvNHR2 02E1) were characterized by rapid seroconversion following infection. Antibody levels to 2 antigens (Ov7 and OvALT-1) increased gradually over the course of infection. Antibody levels to 4 antigens (OvTPX-2, OvL3Chitinase, Ov103 and Ov9m) reached maximum levels coincident with the onset of patency. The levels to 3 antigens (OvProalf C50, OvAldolase, Ov39) varied little over the course of infection. Responses to antigens with functional similarities (OvSOD1, OvSOD2 and OvSOD3 or OvGST1 and OvGST2) showed comparable temporal profiles. This study demonstrates the high degree of immunological cross-reactivity between the antigens of O. volvulus and O. ochengi. The immunogenicity of antigens varied over the course of infection in an antigen-specific manner, which not always reflected developmentally regulated expression of the corresponding gene, possibly owing to cross-reactive epitopes on distinct parasite products.

Item Type: Article
Authors :
NameEmailORCID
Graham, SPs.graham@surrey.ac.ukUNSPECIFIED
Wu, YUNSPECIFIEDUNSPECIFIED
Henkle-Duehrsen, KUNSPECIFIEDUNSPECIFIED
Lustigman, SUNSPECIFIEDUNSPECIFIED
Unnasch, TRUNSPECIFIEDUNSPECIFIED
Braun, GUNSPECIFIEDUNSPECIFIED
Williams, SAUNSPECIFIEDUNSPECIFIED
McCarthy, JUNSPECIFIEDUNSPECIFIED
Trees, AJUNSPECIFIEDUNSPECIFIED
Bianco, AEUNSPECIFIEDUNSPECIFIED
Date : December 1999
Uncontrolled Keywords : Animals, Antibodies, Helminth, Antigens, Helminth, Cattle, Cross Reactions, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Onchocerca volvulus, Onchocerciasis, Recombinant Proteins, Vaccines, Synthetic
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:22
Last Modified : 17 May 2017 14:49
URI: http://epubs.surrey.ac.uk/id/eprint/827519

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800