Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses.

Mwangi, DM, Honda, Y, Graham, SP, Pelle, R, Taracha, EL, Gachanja, J, Nyanjui, JK, Bray, J, Palmer, GH, Brown, WC et al and Mwangi, W (2011) Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses. Vet Immunol Immunopathol, 140 (3-4). pp. 244-251.

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Abstract

Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4(+) and CD8(+) T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p<0.05). The study demonstrated the potential of this technology to elicit significant MHC class II and class I restricted IFN-γ-secreting CD4(+) and CD8(+) T cells to defined vaccine candidate antigens in a natural host, but also underscores the need to improve strategies for eliciting protective CTL responses.

Item Type:	Article
Authors :	Name Email ORCID Mwangi, DM Honda, Y Graham, SP s.graham@surrey.ac.uk Pelle, R Taracha, EL Gachanja, J Nyanjui, JK Bray, J Palmer, GH Brown, WC Mwangi, W
Date :	15 April 2011
DOI :	10.1016/j.vetimm.2010.12.013
Uncontrolled Keywords :	Adjuvants, Immunologic, Animals, Antigens, Protozoan, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cattle, Granulocyte-Macrophage Colony-Stimulating Factor, Interferon-gamma, Lymphocyte Activation, Membrane Proteins, Protozoan Vaccines, Recombinant Proteins, T-Lymphocytes, Cytotoxic, Theileria parva, Theileriasis, Vaccines, DNA
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:22
Last Modified :	16 Jan 2019 17:58
URI:	http://epubs.surrey.ac.uk/id/eprint/827509

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