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Inhibition of adipose tissue lipolysis increases intramuscular lipid use in type 2 diabetic patients.

van Loon, LJ, Manders, RJ, Koopman, R, Kaastra, B, Stegen, JH, Gijsen, AP, Saris, WH and Keizer, HA (2005) Inhibition of adipose tissue lipolysis increases intramuscular lipid use in type 2 diabetic patients. Diabetologia, 48 (10). pp. 2097-2107.

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Abstract

AIMS/HYPOTHESIS: In the present study, we investigated the consequences of adipose tissue lipolytic inhibition on skeletal muscle substrate use in type 2 diabetic patients. MATERIALS AND METHODS: We studied ten type 2 diabetic patients under the following conditions: (1) at rest; (2) during 60 min of cycling exercise at 50% of maximal workload capacity and subsequent recovery. Studies were done under normal, fasting conditions (control trial: CON) and following administration of a nicotinic acid analogue (low plasma non-esterified fatty acid trial: LFA). Continuous [U-13C]palmitate and [6,6 -2H2]glucose infusions were applied to quantify plasma NEFA and glucose oxidation rates, and to estimate intramuscular triacylglycerol (IMTG) and glycogen use. Muscle biopsies were collected before and after exercise to determine net changes in lipid and glycogen content specific to muscle fibre type. RESULTS: Following administration of the nicotinic acid analogue (Acipimox), the plasma NEFA rate of appearance was effectively reduced, resulting in lower NEFA concentrations in the LFA trial (p<0.001). Plasma NEFA oxidation rates were substantially reduced at rest, during exercise and subsequent recovery in the LFA trial. The lower plasma NEFA oxidation rates were compensated by an increase in IMTG and endogenous carbohydrate use (p<0.05). Plasma glucose disposal rates did not differ between trials. In accordance with the tracer data, a greater net decline in type I muscle fibre lipid content was observed following exercise in the LFA trial (p<0.05). CONCLUSIONS/INTERPRETATION: This study shows that plasma NEFA availability regulates IMTG use, and that adipose tissue lipolytic inhibition, in combination with exercise, could be an effective means of augmenting intramuscular lipid and glycogen use in type 2 diabetic patients in an overnight fasted state.

Item Type: Article
Authors :
NameEmailORCID
van Loon, LJUNSPECIFIEDUNSPECIFIED
Manders, RJr.manders@surrey.ac.ukUNSPECIFIED
Koopman, RUNSPECIFIEDUNSPECIFIED
Kaastra, BUNSPECIFIEDUNSPECIFIED
Stegen, JHUNSPECIFIEDUNSPECIFIED
Gijsen, APUNSPECIFIEDUNSPECIFIED
Saris, WHUNSPECIFIEDUNSPECIFIED
Keizer, HAUNSPECIFIEDUNSPECIFIED
Date : October 2005
Identification Number : 10.1007/s00125-005-1889-x
Uncontrolled Keywords : Adipose Tissue, Aged, Algorithms, Body Composition, Body Mass Index, Breath Tests, Diabetes Mellitus, Type 2, Diet, Energy Metabolism, Exercise Test, Fatty Acids, Nonesterified, Female, Glycogen, Humans, Hypolipidemic Agents, Insulin, Kinetics, Lipolysis, Male, Middle Aged, Muscle, Skeletal, Oxidation-Reduction, Palmitates, Pyrazines
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:22
Last Modified : 17 May 2017 14:49
URI: http://epubs.surrey.ac.uk/id/eprint/827505

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