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In vitro effects of Yunnan Baiyao on canine hemangiosarcoma cell lines.

Wirth, KA, Kow, K, Salute, ME, Bacon, NJ and Milner, RJ (2014) In vitro effects of Yunnan Baiyao on canine hemangiosarcoma cell lines. Vet Comp Oncol.

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Abstract

Yunnan Baiyao is a Chinese herbal medicine that has been utilized for its anti-inflammatory, haemostatic, wound healing and pain relieving properties in people. It has been utilized in the veterinary profession to control bleeding in dogs with hemangiosarcoma (HSA) and has been anecdotally reported to prolong survival times in dogs with this neoplasm. This study evaluated the in vitro activity of Yunnan Baiyao against three canine HSA cell lines after treatment with increasing concentrations of Yunnan Baiyao (50, 100, 200, 400, 600 and 800 µg mL(-1) ) at 24, 48 and 72 h. Mean half maximum inhibitory concentration (IC50 ) at 72 h for DEN, Fitz, SB was 369.9, 275.9 and 325.3 µg mL(-1) , respectively. Caspase-3/7 activity increased in correlation with the IC50 in each cell line which was confirmed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, APO-BRDU Kit; BD Biosciences, San Jose, CA, USA) assay. VEGF in cell supernatant was also quantified. Overall, the study found that Yunnan Baiyao causes dose and time dependent HSA cell death through initiation of caspase-mediated apoptosis, which supports future studies involving Yunnan Baiyao.

Item Type: Article
Authors :
NameEmailORCID
Wirth, KAUNSPECIFIEDUNSPECIFIED
Kow, KUNSPECIFIEDUNSPECIFIED
Salute, MEUNSPECIFIEDUNSPECIFIED
Bacon, NJn.bacon@surrey.ac.ukUNSPECIFIED
Milner, RJUNSPECIFIEDUNSPECIFIED
Date : 29 June 2014
Identification Number : https://doi.org/10.1111/vco.12100
Uncontrolled Keywords : Chinese herbal medicine, Yunnan Baiyao, canine hemangiosarcoma
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:19
Last Modified : 17 May 2017 14:48
URI: http://epubs.surrey.ac.uk/id/eprint/827300

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