University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Characterisation of Na+ × H+ exchange in isolated bovine articular chondrocytes

Wilkins, RJ, Mobasheri, A and Hall, AC (1996) Characterisation of Na+ × H+ exchange in isolated bovine articular chondrocytes FASEB Journal, 10 (3).

Full text not available from this repository.

Abstract

We have previously shown that intracellular pH (pH,) in the articular chondrocyte is regulated by a single mechanism, Na+ Hexchange. HCO3-dependent mechanisms play no role, possibly because of the low numbers of anions in the extracellular matrix, and the uptake of SO,2' and Zn2 by modified anion exchangers. Since pH, can modify matrix metabolism, regulation of Nax Hexchange will have important effects on cartilage integrity. We have therefore performed further experiments to characterise the properties of the chondrocyte Nax Hexchange. Recovery from ammonium-induced intracellular acidosis is 'smart', showing allosteric modification with increasing levels of intracellular acidity, sensitive to amiloride (K0.5 = SμM) and is stimulated by cell shrinkage, exposure to serum, and by application of hydrostatic pressure, seemingly by changes to Nax Hexchange phosphorylation state. These properties are consistent with the NHE-1 isoform of Nax Hexchange. However, using antibodies directed against Nax Hproteins we find that both NHE-1 and NHE-3 are present in these cells. Funded by The Wellcome Trust, UK.

Item Type: Article
Authors :
NameEmailORCID
Wilkins, RJUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Hall, ACUNSPECIFIEDUNSPECIFIED
Date : 1 December 1996
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:17
Last Modified : 17 May 2017 14:48
URI: http://epubs.surrey.ac.uk/id/eprint/827163

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800