University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Post-genomic applications of tissue microarrays: basic research, prognostic oncology, clinical genomics and drug discovery.

Mobasheri, A, Airley, R, Foster, CS, Schulze-Tanzil, G and Shakibaei, M (2004) Post-genomic applications of tissue microarrays: basic research, prognostic oncology, clinical genomics and drug discovery. Histol Histopathol, 19 (1). pp. 325-335.

Full text not available from this repository.

Abstract

Tissue microarrays (TMAs) are an ordered array of tissue cores on a glass slide. They permit immunohistochemical analysis of numerous tissue sections under identical experimental conditions. The arrays can contain samples of every organ in the human body, or a wide variety of common tumors and obscure clinical cases alongside normal controls. The arrays can also contain pellets of cultured tumor cell lines. These arrays may be used like any histological section for immunohistochemistry and in situ hybridization to detect protein and gene expression. This new technology will allow investigators to analyze numerous biomarkers over essentially identical samples, develop novel prognostic markers and validate potential drug targets. The ability to combine TMA technology with DNA microarrays and proteomics makes it a very attractive tool for analysis of gene expression in clinically stratified tumor specimens and relate expression of each particular protein with clinical outcome. Public domain software allows researchers to examine digital images of individual histological specimens from TMAs, evaluate and score them and store the quantitative data in a relational database. TMA technology may be specifically applied to the profiling of proteins of interest in other pathophysiological conditions such as congestive heart failure, renal disease, hypertension, diabetes, cystic fibrosis and neurodegenerative disorders. This review is intended to summarize the strengths and weaknesses of TMA technology which will have an increasingly important role in the laboratories of the post-genomic era.

Item Type: Article
Authors :
NameEmailORCID
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Airley, RUNSPECIFIEDUNSPECIFIED
Foster, CSUNSPECIFIEDUNSPECIFIED
Schulze-Tanzil, GUNSPECIFIEDUNSPECIFIED
Shakibaei, MUNSPECIFIEDUNSPECIFIED
Date : January 2004
Uncontrolled Keywords : Animals, Gene Expression Regulation, Neoplastic, Genomics, Humans, Immunohistochemistry, In Situ Hybridization, Internet, Models, Biological, Neoplasms, Oligonucleotide Array Sequence Analysis, Prognosis, Research
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:16
Last Modified : 17 May 2017 14:48
URI: http://epubs.surrey.ac.uk/id/eprint/827129

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800