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Human articular chondrocytes, synoviocytes and synovial microvessels express aquaporin water channels; upregulation of AQP1 in rheumatoid arthritis.

Trujillo, E, González, T, Marín, R, Martín-Vasallo, P, Marples, D and Mobasheri, A (2004) Human articular chondrocytes, synoviocytes and synovial microvessels express aquaporin water channels; upregulation of AQP1 in rheumatoid arthritis. Histol Histopathol, 19 (2). pp. 435-444.

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Abstract

Recent studies have shown that aquaporin water channels are expressed in human Meckel's cartilage. The aim of the present investigation was to determine if human articular chondrocytes and synoviocytes express aquaporin 1 (AQP1) water channels and to establish if there are any alterations in AQP1 expression in osteoarticular disorders such as osteoarthritis (OA) and rheumatoid arthritis (RA). Immunohistochemistry was employed semi-quantitatively to compare the expression of AQP1 in human chondrocytes derived from normal, OA and RA joints. PCR, cloning and sequencing confirmed the presence of AQP1 transcripts in chondrocytes. Normal human tissue microarrays including samples of kidney, choroid plexus and pancreas were used as positive controls for AQP1 expression. In most tissues AQP1 was expressed along endothelial barriers. In the kidney AQP1 was present in the glomerular capillary endothelium, proximal tubule and descending thin limbs. AQP1 was also localized to pancreatic ducts and acini and the apical membrane domain of the choroid plexus. Immunohistochemistry showed that AQP1 is expressed in synovial micro-vessels, synoviocytes and predominantly in chondrocytes located in the deep zone of articular cartilage. Image analysis of normal, OA and RA cartilage suggested that AQP1 may be upregulated in RA. This is the first report of AQP1 mRNA and protein expression in articular chondrocytes and synoviocytes. These findings suggest a potential role for AQP1 and possibly other members of the AQP gene family in the movement of extracellular matrix and metabolic water across the membranes of chondrocytes and synoviocytes for the purposes of chondrocyte volume regulation and synovial homeostasis.

Item Type: Article
Authors :
NameEmailORCID
Trujillo, EUNSPECIFIEDUNSPECIFIED
González, TUNSPECIFIEDUNSPECIFIED
Marín, RUNSPECIFIEDUNSPECIFIED
Martín-Vasallo, PUNSPECIFIEDUNSPECIFIED
Marples, DUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Date : April 2004
Uncontrolled Keywords : Aquaporin 1, Aquaporins, Arthritis, Rheumatoid, Blood Group Antigens, Cartilage, Chondrocytes, Cloning, Molecular, DNA Primers, DNA, Complementary, Humans, Immunohistochemistry, Microscopy, Confocal, Microscopy, Fluorescence, Osteoarthritis, Polymerase Chain Reaction, RNA, Messenger, Synovial Fluid, Synovial Membrane, Tissue Distribution, Up-Regulation
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:16
Last Modified : 17 May 2017 14:48
URI: http://epubs.surrey.ac.uk/id/eprint/827128

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