Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes.
Mobasheri, A, Trujillo, E, Bell, S, Carter, SD, Clegg, PD, Martín-Vasallo, P and Marples, D (2004) Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes. Vet J, 168 (2). pp. 143-150.
Full text not available from this repository.Abstract
Chondrocytes exist in an unusual and highly variable ionic and osmotic environment in the extracellular matrix of articular cartilage. Alterations to the ionic and osmotic environment of chondrocytes influence the volume and ionic content of the cells, which, in turn, modifies the rate at which extracellular matrix macromolecules are synthesized and degraded. Thus, regulation of the water and solute content of chondrocytes will profoundly affect their anabolic and catabolic functions. The water content of cells is effectively influenced by the abundance of aquaporin (AQP) water channels. Recent studies have shown that several AQP water channel isoforms are expressed in chondrocytes from Meckel's cartilage, developing teeth and other orofacial tissues. The aim of the present investigation was to determine if chondrocytes from equine articular cartilage express AQP water channels. Polyclonal antibodies to AQP1, AQP2 and AQP3 were used in conjunction with immunohistochemistry, immunoblotting and quantitative flow cytometry to determine if AQP1, AQP2 and AQP3 are expressed in equine articular chondrocytes. Our studies show that AQP1 and AQP3 are expressed by chondrocytes in articular cartilage in situ and in isolated chondrocytes. We found no evidence for expression of AQP2, the vasopressin-regulated water channel in chondrocytes. AQP1 and AQP3 may be involved in the transport of water and small solutes and osmotically active metabolites across the chondrocyte plasma membrane during volume regulatory behaviour. AQP1 may be involved in transporting metabolic water. AQP3 may participate in the transport of glycerol and structurally related molecules.
Item Type: | Article | ||||||||||||||||||||||||
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Date : | September 2004 | ||||||||||||||||||||||||
DOI : | 10.1016/j.tvjl.2003.08.001 | ||||||||||||||||||||||||
Uncontrolled Keywords : | Animals, Aquaporins, Blotting, Western, Cartilage, Articular, Chondrocytes, Flow Cytometry, Horses, Immunohistochemistry | ||||||||||||||||||||||||
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Depositing User : | Symplectic Elements | ||||||||||||||||||||||||
Date Deposited : | 17 May 2017 10:16 | ||||||||||||||||||||||||
Last Modified : | 16 Jan 2019 17:56 | ||||||||||||||||||||||||
URI: | http://epubs.surrey.ac.uk/id/eprint/827124 |
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