University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Regeneration influences expression of the Na+, K+-atpase subunit isoforms in the rat peripheral nervous system.

Arteaga, MF, Gutiérrez, R, Avila, J, Mobasheri, A, Díaz-Flores, L and Martín-Vasallo, P (2004) Regeneration influences expression of the Na+, K+-atpase subunit isoforms in the rat peripheral nervous system. Neuroscience, 129 (3). pp. 691-702.

Full text not available from this repository.

Abstract

Neural injury triggers changes in the expression of a large number of gene families. Particularly interesting are those encoding proteins involved in the generation, propagation or restoration of electric potentials. The expression of the Na+, K+-ATPase subunit isoforms (alpha, beta and gamma) was studied in dorsal root ganglion (DRG) and sciatic nerve of the rat in normal conditions, after axotomy and during regeneration. In normal DRG, alpha1 and alpha2 are expressed in the plasma membrane of all cell types, while there is no detectable signal for alpha3 in most DRG cells. After axotomy, alpha1 and alpha2 expression decreases evenly in all cells, while there is a remarkable onset in alpha3 expression, with a peak about day 3, which gradually disappears throughout regeneration (day 7). beta1 Is restricted to the nuclear envelope and plasma membrane of neurons and satellite cells. Immediately after injury, beta1 shows a homogeneous distribution in the soma of neurons. No beta2 expression was found. Beta3 Specific immunofluorescence appears in all neurons, although it is brightest in the smallest, diminishing progressively after injury until day 3 and, thereafter, increasing in intensity, until it reaches normal levels. FXYD7 is expressed weakly in a few DRG neurons (less than 2%) and Schwann cells. It increases intensely in satellite cells immediately after axotomy, and in all cell types at day 3. Transient switching of members of the Na+, K+-ATPase isoform family elicited by axotomy suggests variations in the sodium pump isozymes with different affinities for Na+, K+ and ATP from those in intact nerve. This adaptation may be important for regeneration.

Item Type: Article
Authors :
NameEmailORCID
Arteaga, MFUNSPECIFIEDUNSPECIFIED
Gutiérrez, RUNSPECIFIEDUNSPECIFIED
Avila, JUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Díaz-Flores, LUNSPECIFIEDUNSPECIFIED
Martín-Vasallo, PUNSPECIFIEDUNSPECIFIED
Date : 2004
Identification Number : https://doi.org/10.1016/j.neuroscience.2004.08.041
Uncontrolled Keywords : Animals, Axons, Axotomy, Diagnostic Imaging, Fluorescent Antibody Technique, GAP-43 Protein, Ganglia, Spinal, Gene Expression Regulation, Nerve Regeneration, Neurons, Phosphopyruvate Hydratase, Propidium, Protein Isoforms, Rats, Rats, Sprague-Dawley, S100 Proteins, Schwann Cells, Sciatic Nerve, Sodium-Potassium-Exchanging ATPase, Time Factors
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:16
Last Modified : 17 May 2017 14:48
URI: http://epubs.surrey.ac.uk/id/eprint/827121

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800